![Western Blot: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Analysis of 25 ug of mouse brain (lane 1) and C2C12 (lane 2) lysates.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/13006_uNOS%2520Antibody%2520(NOS-3F7-B11%2520B5)-Western%2520Blot-NB120-2801-img0005.jpg)
Western Blot: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Analysis of 25 ug of mouse brain (lane 1) and C2C12 (lane 2) lysates.
![Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a uNOS monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/13007_uNOS-Antibody-(NOS-3F7-B11-B5)-Immunocytochemistry-Immunofluorescence-NB120-2801-img0004.jpg)
Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a uNOS monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
![Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/13008_uNOS-Antibody-(NOS-3F7-B11-B5)-Immunocytochemistry-Immunofluorescence-NB120-2801-img0001.jpg)
Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.
![Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody (NOS-3F7-B11 B5) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/13009_uNOS-Antibody-(NOS-3F7-B11-B5)-Immunocytochemistry-Immunofluorescence-NB120-2801-img0002.jpg)
Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody (NOS-3F7-B11 B5) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.
![Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/13010_uNOS-Antibody-(NOS-3F7-B11-B5)-Immunocytochemistry-Immunofluorescence-NB120-2801-img0003.jpg)
Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.