HEK293 human embryonic kidney cell line transfected with either (A) mouse CD63 or (B) irrelevant transfectants and eGFP were stained with Rat Anti-Mouse CD63 Monoclonal Antibody (Catalog # MAB5417) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005). View our protocol for Staining Membrane-associated Proteins.
CD63 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD63 Monoclonal Antibody (Catalog # MAB5417) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.