Western blot shows lysates of D3 mouse embryonic stem cell line, P19 mouse embryonal carcinoma cell line, C2C12 mouse myoblast cell line, and beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse TCF‑3/E2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7650) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TCF‑3/E2A at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.