![Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ug and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for Fluorescent Western blotting diluted to 1:10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_5/13959_beta%2520Galactosidase%2520Antibody%2520%255BFITC%255D-Western%2520Blot-NB120-6641-img0005.jpg)
Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ug and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for Fluorescent Western blotting diluted to 1:10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M.
![Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Shows detection of a band at ~117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_5/13960_beta%2520Galactosidase%2520Antibody%2520%255BFITC%255D-Western%2520Blot-NB120-6641-img0003.jpg)
Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Shows detection of a band at ~117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
![Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Lane 1 shows 80 ug and lane 2 shows 20 ug loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. Blots were blocked overnight at 4C with blocking buffer for Fluorescent Western blotting conjugated Gt-a-anti-Rabbit IgG was used for detection. Molecular weight estimation was made by comparison to a prestained MW marker (center).in lane M.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_5/13961_beta%2520Galactosidase%2520Antibody%2520%255BFITC%255D-Western%2520Blot-NB120-6641-img0004.jpg)
Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Lane 1 shows 80 ug and lane 2 shows 20 ug loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. Blots were blocked overnight at 4C with blocking buffer for Fluorescent Western blotting conjugated Gt-a-anti-Rabbit IgG was used for detection. Molecular weight estimation was made by comparison to a prestained MW marker (center).in lane M.