lacZ

Gene Symbol	lacZ
Entrez Gene	945006
Alt Symbol	ECK0341, JW0335
Species	Escherichia coli
Gene Type	protein-coding
Description	beta-D-galactosidase
Other Description	-
Swissprots	Q2MC80 P00722
Accessions	AAB18068 AAC73447 AEL16982 NP_414878
Top Pathways	Sphingolipid metabolism, Galactose metabolism, Other glycan degradation

β-Gal (14B7) Mouse mAb - 2372 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Mouse
Clonality	Monoclonal
Clone	14B7

Western blot analysis of cells expressing beta-galactosidase (lane 1) or cells without transfection (lane 2), using Beta-Gal (14B7) Mouse mAb.

Anti-beta Galactosidase antibody - ab9361 from Abcam

Host	Chicken
Clonality	Polyclonal

ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4°C. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green).See Abreview

Anti-beta Galactosidase antibody - ab616 from Abcam

Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-beta Galactosidase antibody (ab616) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells transfected with lacZ-overexpressing plasmid at 15 µgSecondaryHRP-conjugated Goat anti-rabbit at 1/5000 dilutiondeveloped using the ECL techniqueObserved band size : 120 kDa (why is the actual band size different from the predicted?)Additional bands at : 90-100 kDa. We are unsure as to the identity of these extra bands.Exposure time : 2 secondsImage courtesy of an anonymous Abreview.See Abreview

Anti-beta Galactosidase antibody - ab12081 from Abcam

Prices	$382.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µgSecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilutionPerformed under reducing conditions.

Anti-beta Galactosidase antibody - ab107114 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Chicken
Clonality	Polyclonal

Anti-beta Galactosidase antibody (ab107114) at 1 µg/ml + B-galactosidase from E. coli at 10 µgSecondaryGoat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-beta Galactosidase antibody - ab106567 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Chicken
Clonality	Polyclonal

All lanes : Anti-beta Galactosidase antibody (ab106567) at 1 µg/mlLane 1 : beta Galactosidase at 0.005 µgLane 2 : beta Galactosidase at 0.025 µg

Anti-beta Galactosidase antibody [2E9] - ab10074 from Abcam

Prices	$368.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2E9

Anti-beta Galactosidase antibody (Biotin) - ab6645 from Abcam

Prices	$395.00
Sizes	1 mg
Host	Rabbit
Clonality	Polyclonal

Anti-beta Galactosidase antibody (Biotin) - ab182911 from Abcam

Prices	$372.00
Sizes	1 ml
Host	Rabbit
Clonality	Polyclonal

Anti-beta Galactosidase antibody (HRP) - ab191357 from Abcam

Prices	$372.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

beta Galactosidase Antibody - NBP1-45135 from Novus Biologicals

Prices	$279.00
Sizes	500 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence: beta Galactosidase Antibody [NBP1-45135] - MB GABAergic pathway was severely compromised in the double and compound mutants. Expression of GABAergic-specific genes on the coronal sections of E12.5 in wild-type and single knockout embryos (A), as well as in compound and double knockout mutants (B). MB GABAergic induction occurred in double heterozygous mice based on the presence of GABAergic markers (Gad67 and Tal1). (A-E) The GABA phenotype observed at E12.5 in the vlMB of Mgn-/-Mash1-/- (--/--) mice (C2 and D3) was recovered in Mgn+/-Mash1+/- mice (D2 and E). The intermediate zone of m3 domain is magnified in E. GABAn of double heterozygous embryos were maintained at E18.5 (F) and until postnatal death P0 (G). DAPI, NKX2-2, TH, and beta-Gal staining were used as morphological markers). Arrows indicate the loss of postmitotic NKX2-2 neuronal subpopulation in the m2 domain, but not in the m4 domain (C). Scale bars: 500 um in A and G, and 50 um in E.

beta Galactosidase Antibody [FITC] - NB120-6641 from Novus Biologicals

Prices	$229.00
Sizes	1 mg
Host	Rabbit
Clonality	Polyclonal

Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ug and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for Fluorescent Western blotting diluted to 1:10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M.

Anti-Beta-Gal antibody produced in rabbit - SAB2700176 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Beta d Galactosidase Antibody (OAMA01231) - OAMA01231 from Aviva Systems Biology

Prices	$280.00
Sizes	1 mg
Host	Mouse
Clonality	Monoclonal
Clone	'2E9

Anti-beta Galactosidase Antibody - AC20-0150 from Abcore

Prices	$599.00
Sizes	500 µg
Host	Rabbit
Clonality	Polyclonal

Beta-galactosidase tag antibody - AM26630AF-N from Acris Antibodies

Prices	$290.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	5A3

Immunohistochemical detection of beta-galactosidase on paraffin embedded section of pCDNA3-LacZ/293T cells with AM26630AF-N.

Beta-galactosidase tag antibody - AP21258AF-N from Acris Antibodies

Prices	$235.00
Sizes	10 mg
Host	Rabbit
Clonality	Polyclonal

Beta-galactosidase tag antibody - AP21258BT-N from Acris Antibodies

Prices	$370.00
Sizes	1 ml
Host	Rabbit
Clonality	Polyclonal

Beta-galactosidase tag antibody - AP21258PU-N from Acris Antibodies

Prices	$500.00
Sizes	1 mg
Host	Rabbit
Clonality	Polyclonal

Beta-galactosidase tag antibody - AP31768PU-N from Acris Antibodies

Prices	$340.00
Sizes	400 µl
Host	Chicken
Clonality	Polyclonal

Beta-galactosidase tag antibody - R1064 from Acris Antibodies

Prices	$440.00
Sizes	2 ml
Host	Rabbit
Clonality	Polyclonal

Figure 1. Western blotting using Fluorescein conjugated anti-Beta-galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to Nitrocellulose and blocking using blocking buffer for fluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase (Cat.-No. R1064F) diluted to 1/10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® Imaging System. Other detection systems will yield similar results.

Beta-galactosidase tag antibody - R1064BS from Acris Antibodies

Prices	$450.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of rabbit anti-Beta-galactosidase (E. coli) antibody biotin conjugated. Lane 1: Beta-galactosidase. Load: 50 ng per lane. Primary antibody: Beta-galactosidase (E. coli) antibody biotin conjugated Cat.-No. R1064B) at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer ( for fluorescent Western blotting) for 30 min at RT. Predicted/observed size: 116 kDa, 116 kDa for Beta-galactosidase.

Beta-galactosidase tag antibody - R1064FS from Acris Antibodies

Prices	$450.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blotting using fluorescein conjugated anti-Beta-galactosidase tag antibody shows a band at ~117 kDa (lanes 1-3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for bluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase Cat.-No. R1064F diluted to 1/10,000. Reaction occurred for 2 hours at RT. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® imaging system. Other detection systems will yield similar results.

Beta-galactosidase tag antibody - R1064HRPS from Acris Antibodies

Prices	$450.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Beta-galactosidase tag antibody - R1064PS from Acris Antibodies

Prices	$450.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blotting using Beta-galactosidase antibody Cat.-No R1064PS. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for N<em>on-Reducing Conditions</em> of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under <em>Reducing Conditions</em> are shown on the right. Blots were blocked overnight at 4°C with blocking buffer for fluorescent Western blotting (p/n MB-070). The membrane was probed with Beta-galactosidase antibody Cat.-No R1064P diluted to 1/10,000. Reaction occurred overnight at 4°C. Dylight649™ conjugated goat-anti-rabbit IgG was used for detection. Molecular weight estimation was made by comparison to a prestained MW marker (center). Fluorescence image was captured using the VersaDoc® imaging system developed by BIO-RAD. Other detection systems will yield similar results.

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