Western blot analysis of G6PC Antibody (Center) (Cat. #AP5224c) in K562 cell line lysates (35ug/lane).G6PC (arrow) was detected using the purified Pab.
G6PC Antibody (Center) (Cat. #AP5224c) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
G6PC Antibody (Center) (Cat. #AP5224c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.