Anti-Actin antibody [EPR16770]

• Supplier: Abcam
• Catalog: ab198991
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16770
• Applications: WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Actin aa 300 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: ACTA1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Actin antibody [EPR16770] (ab198991) at 1/20000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : 293 (Human embryonic kidney) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Actin antibody [EPR16770] (ab198991) at 1/20000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal heart lysateLane 3 : Human fetal kidney lysateLane 4 : Human fetal spleen lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-Actin antibody [EPR16770] (ab198991) at 1/20000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Mouse spleen lysateLane 5 : Rat brain lysateLane 6 : Rat heart lysateLane 7 : Rat kidney lysateLane 8 : Rat spleen lysateLane 9 : C6 (Rat glial tumor cells) whole cell lysateLane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Actin was immunoprecipitated from 1mg of 293 (Human embryonic kidney) whole cell extract with ab198991 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198991 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: 293 whole cell extract 10 µg (Input). Lane 2: ab198991 IP in 293 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198991 in 293 whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.