ACTA1

Gene Symbol ACTA1
Entrez Gene 58
Alt Symbol ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3
Species Human
Gene Type protein-coding
Description actin, alpha 1, skeletal muscle
Other Description actin, alpha skeletal muscle|nemaline myopathy type 3
Swissprots P68133 Q5T8M9
Accessions AAA60296 AAF02694 EAW69898 P68133 AK096902 BAG53395 AK300697 BAH13327 AL598491 AY280960 AAP37280 BC012597 AAH12597 BX648545 CR536516 CAG38754 CR541796 CAG46595 DQ892949 ABM83875 DQ896197 ABM87196 J00068 AAB59376 NM_001100 NP_001091
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Location Cytoplasm, cytoskeleton.

Pan-Actin (D18C11) Rabbit mAb - 8456 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D18C11
Western blot analysis of extracts from various cell lines using Pan-Actin (D18C11) Rabbit mAb.

Pan-Actin Antibody - 4968 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, L929, C6 and COS cells, using Pan-Actin Antibody.

Actin (I-19) - sc-1616 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Actin (I-19): sc-1616. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal fluorescein immunostaining of actin filaments. Note nuclear rhodamine immunostaining with PCNA (PC-10): sc-56.

Actin (C-2) - sc-8432 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Actin (C-2): sc-8432. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.

Actin (H-196) - sc-7210 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Actin (H-196): sc-7210. Western blot analysis of Actin expression in A-10 whole cell lysate.

Actin (H-300) - sc-10731 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Actin (H-300): sc-10731. Western blot analysis of Actin expression in Sol8 whole cell lysate.

Actin (NH3) - sc-58679 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Actin (NH3): sc-58679. Western blot analysis of Actin expression in A-10 whole cell lysate.

Actin (C-11) - sc-1615 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Actin (C-11): sc-1615. Western blot analysis of Actin expression in C32 (A), BC<sub>3</sub>H1 (B), Sol8 (C), HeLa (D), KNRK (E) and NIH/3T3 (F) whole cell lysates.

Actin (H-6) - sc-376421 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Actin (H-6): sc-376421. Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic staining of smooth muscle cells.

Actin (SPM161) - sc-56459 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Actin (SPM161): sc-56459. Western blot analysis of Actin expression in HeLa (A), Sol8 (B) and C32 (C) whole cell lysates.

Actin (8.S2.1) - sc-70319 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Actin (8.S2.1): sc-70319. Western blot analysis of Actin expression in NIH/3T3 (A) and HL-60 (B) whole cell lysates.

α-Actin (0.N.5) - sc-58669 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-Actin (0.N.5): sc-58669. Western blot analysis of α-Actin expression in A-673 (A) and Sol8 (B) whole cell lysates.

α-Actin (Alpha Sr-1) - sc-58671 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-Actin (Alpha Sr-1): sc-58671. Western blot analysis of α-Actin expression in rat skeletal muscle (A) and mouse skeletal muscle (B) tissue extracts.

α-Actin (G-12) - sc-130619 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
a-Actin (G-12): sc-130619. Western blot analysis of a-actin expression in HeLa whole cell lysate (A) and rabbit muscular (A), fish (B) and rat brain (C) tissue extracts.

Smooth Muscle Actin (CGA7) - sc-53015 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smooth Muscle Actin (CGA7): sc-53015. Western blot analysis of Smooth Muscle Actin expression in A-10 whole cell lysate.

Anti-Actin antibody - ab52220 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-Actin antibody (ab52220) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HeLa cell extract with immunizing peptide

Anti-Actin antibody - ab95437 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Actin antibody (ab95437) at 0.5 µg/mlLane 1 : Rat cardiac muscle missue lysateLane 2 : Rat brain tissue lysateLane 3 : Rat testis tissue lysateLane 4 : Rat skeletal muscle tissue lysateLane 5 : MM231 cell lysateLane 6 : HeLa cell lysateLane 7 : SMMC cell lysateLane 8 : HT1080 cell lysateLane 9 : SW620 cell lysatedeveloped using the ECL technique

Anti-Actin antibody [EPR16770] - ab198991 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16770
All lanes : Anti-Actin antibody [EPR16770] (ab198991) at 1/20000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : 293 (Human embryonic kidney) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Actin antibody [EPR8484] (Alexa Fluor® 488) - ab190196 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8484
ab190196 staining Actin in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab190196 at 1/50 dilution and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat anti-mouse AlexaFluor®594 (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

Anti-Actin antibody [EPR8484] - Loading Control (HRP) - ab185058 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8484
All lanes : Anti-Actin antibody [EPR8484] - Loading Control (HRP) (ab185058) at 1/10000 dilutionLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µgLane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 20 µgLane 3 : Human Fetal Heart at 5 µgLane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 10 µgLane 5 : Skeletal Muscle (Rat) Tissue Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Actin antibody [MAC 237] - ab50591 from Abcam

Prices $382.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone MAC 237
IHC image of Actin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50591, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Actin antibody [Q20-K] (FITC) - ab139548 from Abcam

Prices $376.00
Sizes 50 tests
Host Rabbit
Clonality Monoclonal
Clone Q20-K
Flow cytometry analysis of Human PBMCs, staining Actin with ab139548.Cells were fixed, permeabilized and stained with anti-Human Actin FITC (blue, 10 μl per test) or with an isotype control (black).

Anti-Actin antibody [S12-I] - ab136905 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone S12-I
Anti-Actin antibody [S12-I] (ab136905) at 1/2000 dilution + Mouse brain tissue lysate at 50 µg

Anti-Actin antibody - C-terminal - ab81144 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Alpha Skeletal Muscle Actin antibody - ab52218 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Alpha Skeletal Muscle Actin antibody (ab52218) at 1/500 dilutionLane 1 : extracts from LOVO cells,Lane 2 : extracts from LOVO cells, with immunizing peptide
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