Anti-AIF antibody
| Name | Anti-AIF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab1998 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Peptide corresponding to amino acids 517 to531 of human AIF |
| Description | Rabbit Polyclonal |
| Gene | TXNDC12 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-AIF antibody (ab1998) at 1 µg/mlLane 1 : K562 cell lysate Lane 2 : Rat heart tissue lysateLane 3 : Mouse heart tissue lysate
Immunohistochemistry of AIF in human retina with anti-AIF (IN) at 10 µg/ml.
ab1998 staining AIF in human U2OS cells by Immunocytochemistry/ Immunofluorescence.Samples were fixed using 4% paraformaldehyde.Left image shows fixed cells labeled with ab1998 (red) and cyclophilin A (green).Right image shows U2OS cell 6 hours after induction of apoptosis by 200 nM staurosporine.Note translocated of AIF to the nucleus upon induction of apoptosis.
ICC/IF image of ab1998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1998, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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