Anti-Asialoglycoprotein Receptor 2 antibody [2A12]

• Supplier: Abcam
• Catalog: ab139298
• Prices: $384.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 2A12
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant full length Human Asialoglycoprotein Receptor 2, produced in HEK293T cells
• Description: Mouse Monoclonal
• Gene: ASGR2
• Conjugate: Unconjugated

Product images

ICC/IF image of ab139298 stained HepG2 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab139298 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Mouse IgG (H+L) (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-Asialoglycoprotein Receptor 2 antibody [2A12] (ab139298) at 1/4000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected the pCMV6-ENTRY Asialoglycoprotein Receptor 2 cDNA Lysates/proteins at 5 µg per lane.

Immunohistochemical analysis of Paraffin-embedded Human colon adenocarcinoma tissue labelling Asialoglycoprotein Receptor 2 with ab139298 at 1/150 dilution.

Immunohistochemical analysis of Paraffin-embedded Human liver carcinoma tissue labelling Asialoglycoprotein Receptor 2 with ab139298 at 1/150 dilution.

Immunohistochemical analysis of Paraffin-embedded Human ovary adenocarcinoma tissue labelling Asialoglycoprotein Receptor 2 with ab139298 at 1/150 dilution.

Immunohistochemical analysis of Paraffin-embedded Human thyroid carcinoma tissue labelling Asialoglycoprotein Receptor 2 with ab139298 at 1/150 dilution.

Immunohistochemical analysis of Paraffin-embedded Human endometrium adenocarcinoma tissue labelling Asialoglycoprotein Receptor 2 with ab139298 at 1/150 dilution.

Immunohistochemical analysis of Paraffin-embedded Human lymph node tissue labelling Asialoglycoprotein Receptor 2 with ab139298 at 1/150 dilution.