Anti-ATPB antibody [3D5] - Mitochondrial Marker

• Supplier: Abcam
• Catalog: ab14730
• Prices: $404.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 3D5
• Applications: WB IHC-F ICC/IF ICC/IF IP FC IHC-P
• Species Reactivities: Mouse, Rat, Bovine, Human, C. elegans, Monkey
• Antigen: Whole heart mitochondria (Human)
• Description: Mouse Monoclonal
• Gene: ATP5B
• Conjugate: Unconjugated

Product images

ab14730 staining ATPB in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 0.02% Tween 20) for 1 hour at 21°C. A DyLight® 550-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

ICC/IF image of ab14730 stained human HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14730, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and MCF7 cells.

All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) at 0.8 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

ab14730 (2µg/ml) staining ATPB in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and mitochondrial staining of epithelium.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)Lane 1 : isolated mitochondria from human heart at 5 µgLane 2 : isolated mitochondria from bovine heart at 1 µgLane 3 : isolated mitochondria from rat heart at 10 µgLane 4 : isolated mitochondria from mouse heart at 10 µg

ab14730 staining ATPB in cultured human fibroblasts. Cells were fixed, permeabilized and then labelled with ab14730 followed by an AlexaFluor® 488-conjugated Goat anti-Mouse IgG1-specific secondary antibody (2 µg/ml)

ab14730 staining ATPB in rat liver tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 10% goat serum) for 18 hours at 4°C. An Alexa Fluor® 555-conjugated goat anti-mouse polyclonal (1/500) was used as the secondary antibody.See Abreview

ab14730 (blue) at 1µg/ml staining ATPB in HL-60 cells and analyzed by Flow cytometry. Red histogram represents equal quantity of isotype control.

Product References

Reconstitution of the mitochondrial calcium uniporter in yeast. - Reconstitution of the mitochondrial calcium uniporter in yeast.

Kovacs-Bogdan E, Sancak Y, Kamer KJ, Plovanich M, Jambhekar A, Huber RJ, Myre MA, Blower MD, Mootha VK. Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8985-90. doi:

Genetic deletion of transglutaminase 2 does not rescue the phenotypic deficits - Genetic deletion of transglutaminase 2 does not rescue the phenotypic deficits

Menalled LB, Kudwa AE, Oakeshott S, Farrar A, Paterson N, Filippov I, Miller S, Kwan M, Olsen M, Beltran J, Torello J, Fitzpatrick J, Mushlin R, Cox K, McConnell K, Mazzella M, He D, Osborne GF, Al-Nackkash R, Bates GP, Tuunanen P, Lehtimaki K, Brunner D, Ghavami A, Ramboz S, Park L, Macdonald D, Munoz-Sanjuan I, Howland D. PLoS One. 2014 Jun 23;9(6):e99520.

An uncoupling channel within the c-subunit ring of the F1FO ATP synthase is the - An uncoupling channel within the c-subunit ring of the F1FO ATP synthase is the

Alavian KN, Beutner G, Lazrove E, Sacchetti S, Park HA, Licznerski P, Li H, Nabili P, Hockensmith K, Graham M, Porter GA Jr, Jonas EA. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10580-5. doi:

Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase beta and regulates - Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase beta and regulates

Rahman M, Nirala NK, Singh A, Zhu LJ, Taguchi K, Bamba T, Fukusaki E, Shaw LM, Lambright DG, Acharya JK, Acharya UR. J Cell Biol. 2014 Jul 21;206(2):289-305.

EF-P dependent pauses integrate proximal and distal signals during translation. - EF-P dependent pauses integrate proximal and distal signals during translation.

Elgamal S, Katz A, Hersch SJ, Newsom D, White P, Navarre WW, Ibba M. PLoS Genet. 2014 Aug 21;10(8):e1004553.

Quantitative proteomics analysis identifies mitochondria as therapeutic targets - Quantitative proteomics analysis identifies mitochondria as therapeutic targets

Chen X, Wei S, Ma Y, Lu J, Niu G, Xue Y, Chen X, Yang F. Theranostics. 2014 Sep 18;4(12):1164-75.

Characterization of the mode of action of a potent dengue virus capsid inhibitor. - Characterization of the mode of action of a potent dengue virus capsid inhibitor.

Scaturro P, Trist IM, Paul D, Kumar A, Acosta EG, Byrd CM, Jordan R, Brancale A, Bartenschlager R. J Virol. 2014 Oct;88(19):11540-55.

A truncating PET100 variant causing fatal infantile lactic acidosis and isolated - A truncating PET100 variant causing fatal infantile lactic acidosis and isolated

Olahova M, Haack TB, Alston CL, Houghton JA, He L, Morris AA, Brown GK, McFarland R, Chrzanowska-Lightowlers ZM, Lightowlers RN, Prokisch H, Taylor RW. Eur J Hum Genet. 2014 Oct 8.

MICU2, a paralog of MICU1, resides within the mitochondrial uniporter complex to - MICU2, a paralog of MICU1, resides within the mitochondrial uniporter complex to

Plovanich M, Bogorad RL, Sancak Y, Kamer KJ, Strittmatter L, Li AA, Girgis HS, Kuchimanchi S, De Groot J, Speciner L, Taneja N, Oshea J, Koteliansky V, Mootha VK. PLoS One. 2013;8(2):e55785.

IF1 limits the apoptotic-signalling cascade by preventing mitochondrial - IF1 limits the apoptotic-signalling cascade by preventing mitochondrial

Faccenda D, Tan CH, Seraphim A, Duchen MR, Campanella M. Cell Death Differ. 2013 May;20(5):686-97.