Anti-Blooms Syndrome Protein Blm antibody
| Name | Anti-Blooms Syndrome Protein Blm antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2179 |
| Prices | $398.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP ICC/IF |
| Species Reactivities | Human, Bovine, Dog, Pig, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) |
| Description | Rabbit Polyclonal |
| Gene | BLM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
HeLa cells cultured on coverslips were fixed in 4% paraformaldehyde then stained with ab2179 (green) at a concentration of 5µg/ml. The DNA stained with DAPI is shown in red (100x magnification).
HeLa cells were treated with 15µM Bleomycin for 2 hours to generate DNA double stranded breaks (DSBs). Paraformaldehyde-fixed cells were co-immunofluorescently labelled with anti-gamma H2AX (DNA DSB marker) and anti-BLM (ab2179). ab 2179 was used at a concentration of 5µg/ml. Colocalization at the sites of DNA DSBs is identified by the arrows. In the final panel DAPI is pseudo-coloured blue, while anti-gamma H2AX and anti-Blm are green and red respectively.
Product References
Chk1 protects against chromatin bridges by constitutively phosphorylating BLM - Chk1 protects against chromatin bridges by constitutively phosphorylating BLM
Petsalaki E, Dandoulaki M, Morrice N, Zachos G. J Cell Sci. 2014 Sep 15;127(Pt 18):3902-8.
Replication stress and mitotic dysfunction in cells expressing simian virus 40 - Replication stress and mitotic dysfunction in cells expressing simian virus 40
Hu L, Filippakis H, Huang H, Yen TJ, Gjoerup OV. J Virol. 2013 Dec;87(24):13179-92.
The human RecQ helicases BLM and RECQL4 cooperate to preserve genome stability. - The human RecQ helicases BLM and RECQL4 cooperate to preserve genome stability.
Singh DK, Popuri V, Kulikowicz T, Shevelev I, Ghosh AK, Ramamoorthy M, Rossi ML, Janscak P, Croteau DL, Bohr VA. Nucleic Acids Res. 2012 Aug;40(14):6632-48.
PICH and BLM limit histone association with anaphase centromeric DNA threads and - PICH and BLM limit histone association with anaphase centromeric DNA threads and
Ke Y, Huh JW, Warrington R, Li B, Wu N, Leng M, Zhang J, Ball HL, Li B, Yu H. EMBO J. 2011 Jul 8;30(16):3309-21.