Anti-BNIP3 antibody - Carboxyterminal end

• Supplier: Abcam
• Catalog: ab65874
• Prices: $384.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide: AIGLGIYIGRRLTTSTST corresponding to a sequence near the C-terminal of human BNIP3, identical to the related mouse and rat sequence
• Description: Rabbit Polyclonal
• Gene: BNIP3
• Conjugate: Unconjugated

Product images

Anti-BNIP3 antibody - Carboxyterminal end (ab65874) at 2 µg/ml + rat thymus tissue lysate at 30 µg

ICC/IF image of ab65874 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65874, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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H-ras up-regulates expression of BNIP3. - H-ras up-regulates expression of BNIP3.

Kalas W, Swiderek E, Rapak A, Kopij M, Rak J, Strzadala L. Anticancer Res. 2011 Sep;31(9):2869-75.