BNIP3

Gene Symbol BNIP3
Entrez Gene 664
Alt Symbol NIP3
Species Human
Gene Type protein-coding
Description BCL2/adenovirus E1B 19kDa interacting protein 3
Other Description BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Swissprots O14620 Q96GP0 Q12983
Accessions AAW62256 CAI45349 EAW49143 Q12983 AF002697 AAC16738 AK130264 AK222626 BAD96346 AK295136 BAG58159 BC009342 BC021989 AAH21989 BC067818 AAH67818 BC080643 AAH80643 BX647339 HQ448726 ADQ33204 HY049371 U15174 AAC00022 NM_004052 NP_004043
Function Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. {ECO:0000269|PubMed:19935772, ECO:0000269|PubMed:22292033}.
Subcellular Location Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Note=Coexpression with the EIB 19- kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.
Top Pathways Legionellosis

BNIP3 Antibody - 13795 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of MCF7 and HeLa cells, untreated (-) or cobalt chloride-treated (100 μM, overnight; +), using BNIP3 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

BNIP-3 (ANa40) - sc-56167 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BNIP-3 (ANa40): sc-56167. Western blot analysis of BNIP-3 expression in non-transfected: sc-117752 (A) and mouse BNIP-3 transfected: sc-127225 (B) 293T whole cell lysates.

BNIP-3 (C-18) - sc-1715 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Nix (H-8) - sc-166332 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nix (H-8): sc-166332. Western blot analysis of Nix expression in non-transfected: sc-117752 (A) and human Nix transfected: sc-113558 (B) 293T whole cell lysates.

Nix (D-1) - sc-166114 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nix (D-1): sc-166114. Western blot analysis of Nix expression in non-transfected: sc-117752 (A) and human Nix transfected: sc-113558 (B) 293T whole cell lysates.

Nix (E-1) - sc-166314 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nix (E-1): sc-166314. Western blot analysis of Nix expression in non-transfected: sc-117752 (A) and human Nix transfected: sc-113558 (B) 293T whole cell lysates.

Anti-BNIP3 (phospho S95) antibody - ab83940 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-BNIP3 antibody - ab38621 from Abcam

Prices $390.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BNIP3 antibody (ab38621) at 1/100 dilutionLane 1 : Ramos cell lysateLane 2 : Mouse brain tissue lysate

Anti-BNIP3 antibody - ab134702 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-BNIP3 antibody (ab134702) at 1/500 dilution + K562 cell extract at 30 µg

Anti-BNIP3 antibody [ANa40] - ab10433 from Abcam

Prices $404.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ANa40
Immunohistochemical analysis of Human laryngeal squamous cell carcinoma tissue, staining BNIP3 with ab10433. Antigen retrieval was performed by heat mediation in citrate buffer. The sections were incubated with primary antibody (1/100) overnight at 4°C in a humidified chamber. Staining was visualized using DAB, followed by hematoxylin nuclear counterstaining.

Anti-BNIP3 antibody [ANa40] - ab88729 from Abcam

Prices $373.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone ANa40

Anti-BNIP3 antibody [ANa40] (Alexa Fluor® 647) - ab196706 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ANa40
ab196706 staining BNIP3 in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196706 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-BNIP3 antibody [ANa40] (HRP) - ab196707 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ANa40
Anti-BNIP3 antibody [ANa40] (HRP) (ab196707) at 1/5000 dilution + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-BNIP3 antibody [EPR4034] - ab109362 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4034
All lanes : Anti-BNIP3 antibody [EPR4034] (ab109362) at 1/1000 dilution (unpurified)Lane 1 : Jurkat cell lysate treated with etoposideLane 2 : Jurkat cell lysateLane 3 : SH-SY5Y cell lysate treated with camptothecinLane 4 : SH-SY5Y cell lysateLane 5 : MCF-7 cell lysate treated with Cocl2Lysates/proteins at 10 µg per lane.

Anti-BNIP3 antibody - Carboxyterminal end - ab65874 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-BNIP3 antibody - Carboxyterminal end (ab65874) at 2 µg/ml + rat thymus tissue lysate at 30 µg

Anti-NIP3 / BNIP3 Antibody (aa112-124) IHC-plus™ - LS-B3305 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Anti-BNIP3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3305 concentration 10 ug/ml.

Anti-NIP3 / BNIP3 Antibody (aa2-160) - LS-C35154 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-NIP3 / BNIP3 Antibody (aa1-50) - LS-C77112 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NIP3 / BNIP3 Antibody (Ser60) - LS-C77113 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NIP3 / BNIP3 Antibody (C-Terminus) - LS-C127054 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NIP3 / BNIP3 Antibody (aa1-50, Biotin) - LS-C142865 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NIP3 / BNIP3 Antibody (aa1-50, DY650) - LS-C142866 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NIP3 / BNIP3 Antibody (aa1-50, DY488) - LS-C142867 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NIP3 / BNIP3 Antibody (aa1-50, HRP) - LS-C142868 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NIP3 / BNIP3 Antibody (aa1-50, DY550) - LS-C142869 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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