Anti-BRCA1 antibody [8F7]
| Name | Anti-BRCA1 antibody [8F7] |
|---|---|
| Supplier | Abcam |
| Catalog | ab94 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 8F7 |
| Applications | IHC-P WB IP EMSA |
| Species Reactivities | Human |
| Antigen | Fusion protein, corresponding to amino acids 341-748 of Human BRCA 1 |
| Description | Mouse Monoclonal |
| Gene | BRCA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab94 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
The central region of BRCA1 binds preferentially to supercoiled DNA. - The central region of BRCA1 binds preferentially to supercoiled DNA.
Brazda V, Jagelska EB, Liao JC, Arrowsmith CH. J Biomol Struct Dyn. 2009 Aug;27(1):97-104.
BRCA1 gene expression in breast cancer: a correlative study between real-time - BRCA1 gene expression in breast cancer: a correlative study between real-time
Al-Mulla F, Abdulrahman M, Varadharaj G, Akhter N, Anim JT. J Histochem Cytochem. 2005 May;53(5):621-9.
Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its - Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its
Okada S, Ouchi T. J Biol Chem. 2003 Jan 17;278(3):2015-20. Epub 2002 Nov 8.
BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest. - BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.
Ongusaha PP, Ouchi T, Kim KT, Nytko E, Kwak JC, Duda RB, Deng CX, Lee SW. Oncogene. 2003 Jun 12;22(24):3749-58.