BRCA1

Gene Symbol BRCA1
Entrez Gene 672
Alt Symbol BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
Species Human
Gene Type protein-coding
Description breast cancer 1, early onset
Other Description BRCA1/BRCA2-containing complex, subunit 1|Fanconi anemia, complementation group S|RING finger protein 53|breast and ovarian cancer susceptibility protein 1|breast and ovarian cancer sususceptibility protein 1|breast cancer type 1 susceptibility protein|protein phosphatase 1, regulatory subunit 53
Swissprots O15129 Q7KYU9 Q3LRJ6 Q6IN79 Q3LRJ0 Q1RMC1 P38398
Accessions AAB34725 AAB40910 AAC37594 AAC50330 AAF97939 AAM18218 AAM18219 AAM18220 AAM18221 AAM18222 AAM18223 AAM18224 AAM18225 AAM18226 AAN10167 AAN61423 AAN61424 AAN61425 AAN71744 AAP12647 AAP70031 AAU21563 AAU21564 AAU21565 AAZ05886 AAZ16492 AAZ79406 AAZ79407 AAZ79408 AAZ79409 AAZ79410 ABA29208 ABA29211 ABA29214 ABA29217 ABA29220 ABA29223 ABA29226 ABA29229 ABB87053 ABB87054 ABB87055 ABB87056 ABB87057 ABB87058 ABB87059 ABB87060 ABB87061 ABB87062 ABB87063 ABB87064 ABB87065 ABB87066 ABB87067 ABB87068 ABB87069 ABB87070 ABB87071 ABB87072 ABB87073 ABB87074 ABB87075 ABB87076 ABB87077 ABB87078 ABB87079 ABF14462 ACR33809 AFU88802 AFU88803 AFU88804 AFU88805 AFU88806 AFU88807 AFU88808 AFU88809 AIC83539 AIC83540 AIC83541 AIC83542 AIC83544 AIC83545 AIC83546 AIC83547 AIC83548 AIC83549 AIC83550 AIC83551 AIC83552 AIC83553 AIC83554 AIC83555 AIC83556 AIC83557 AIC83558 AIC83559 AIC83571 AIC83572 AIC83573 AIC83574 AIC83575 AIC83576 AIC83577 AIC83578 AIC83579 AIC83580 AIC83581 AIC83582 AIY34492 AKE50574 AKE50575 AKE50576 AKE50577 AKE50578 AKE50579 AKE50580 AKE50581 CAA70003 CCD57768 CCD57769 CCD57770 CCD57771 CCD57772 CCD57773 CCD57774 EAW60922 EAW60923 EAW60924 EAW60925 EAW60926 EAW60927 EAW60928 EAW60929 EAW60930 EAW60931 EAW60932 EAW60933 EAW60934 EAW60935 EAW60936 EAW60937 EAW60938 EAW60939 EAW60940 EAW60941 EAW60942 P38398 AB621825 BAK64160 AF005068 AAB61673 AF274503 AK307553 AK308084 AK316200 BAH14571 AL701927 AY354539 AAQ92977 AY751490 AAU93634 BC012577 BC030969 AAH30969 BC038947 BC046142 BC062429 AAH62429 BC072418 AAH72418 BC085615 AAH85615 BC106745 AAI06746 BC106746 AAI06747 BC114511 BC114562 BC115037 AAI15038 BU617173 BU679389 DQ333386 ABC59810 DQ333387 ABC59811 DQ363751 ABC88652 JN686490 AEQ98814 U14680 AAA73985 U64805 AAC00049 U68041 AAB08105 Y08864 NM_007294 NP_009225 NM_007297 NP_009228 NM_007298 NP_009229 NM_007299 NP_009230 NM_007300 NP_009231 NR_027676 NM_007295 NM_007296 NM_007301 NM_007302 NM_007303 NM_007305 NM_007306
Function E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by bindi
Subcellular Location Isoform 5: Cytoplasm {ECO:0000269|PubMed:8972225}.
Tissue Specificity Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
Top Pathways Ubiquitin mediated proteolysis, Fanconi anemia pathway, PI3K-Akt signaling pathway, MicroRNAs in cancer

Phospho-BRCA1 (Ser1524) Antibody - 9009 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of untreated and UV-treated (50 mJ/cm2,30 min) HeLa cells and HT-1376 cells, using Phospho-BRCA1 (Ser1524) Antibody (upper) and BRCA1 Antibody #9010 (lower).

BRCA1 Antibody - 9010 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of untreated and UV-treated (50 mJ/cm2, 30 min) HeLa cells, using BRCA1 antibody.

BRCA1 (D54A8) Rabbit mAb - 9025 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D54A8
Western blot analysis of extracts from various cell lines using BRCA1 (D54A8) Rabbit mAb.

BRCA1 (D-9) - sc-6954 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BRCA1 siRNA (h): sc-29219. Western blot analysis of BRCA1 expression in non-transfected control (A) and BRCA1 siRNA transfected (B) A-431 cells. Blot probed with BRCA1 (C-20): sc-642. α-actinin (H-2): sc-17829 used as specificity and loading control.

BRCA1 (D-20) - sc-641 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BRCA1 (D-20): sc-641. Western blot analysis of human recombinant BRCA1 fusion protein.

BRCA1 (GLK-2) - sc-56030 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

BRCA1 (C-20) - sc-642 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BRCA1 (C-20): sc-642. Western blot analysis of BRCA1 expression in A-431 (A), HeLa (B) and NIH/3T3 (C) nuclear extracts.

BRCA1 (H-100) - sc-7867 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BRCA1 (H-100): sc-7867. Western blot analysis of BRCA1 expression in LADMAC whole cell lysate.

BRCA1 (I-20) - sc-646 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BRCA1 (I-20): sc-646. Western blot analysis of BRCA1 expression in A-431 (A), HeLa (B), MCF7 (C), KNRK (D) and NIH/3T3 (E) nuclear extracts.

BRCA1 (K-18) - sc-1021 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BRCA1 (K-18): sc-1021. Western blot analysis of human recombinant BRCA1 fusion protein.

BRCA1 (H-300) - sc-28234 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BRCA1 (H-300): sc-28234. Western blot analysis of BRCA1 expression in LADMAC whole cell lysate.

p-BRCA1 (G-4) - sc-166793 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of BRCA1 phosphorylation in untreated (A,C) and UV irradiated (B,D) HeLa whole cell lysates. Antibodies tested include p-BRCA1 (G-4): sc-166793 (A,B) and BRCA1 (H-300): sc-28234 (C,D).

p-BRCA1 (Ser 1497) - sc-12889 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-BRCA1 (Ser 1497): sc-12889. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

p-BRCA1 (Ser 988) - sc-12888 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-BRCA1 Antibody (Ser 988): sc-12888-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded human vulva/anal skin tissue showing nuclear and cytoplasmic staining of epidermal cells.

p-BRCA1 (Ser 1524) - sc-101648 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-BRCA1 (Ser 1524): sc-101648. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytolasmic staining.

p-BRCA1 (Ser 1423) - sc-101647 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-BRCA1 (Ser 1423): sc-101647. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytolasmic staining.

Anti-BRCA1 (phospho S1189) antibody - ab3542 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-BRCA1 (phospho S1280) antibody - ab3544 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-BRCA1 (phospho S1387) antibody - ab2843 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-BRCA1 (phospho S1423) antibody - ab2838 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-BRCA1 (phospho S1423) antibody - ab47325 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab47325 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47325, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BRCA1 (phospho S1423) antibody - ab194753 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BRCA1 (phospho S1423) antibody (ab194753) at 1/500 dilutionLane 1 : HT29 cell extract, untreatedLane 2 : HT29 cell extract, treated with anisomycin

Anti-BRCA1 (phospho S1457) antibody - ab138407 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-BRCA1 (phospho S1457) antibody (ab138407) at 1/500 dilution + Extracts from 293 cells treated with epo 20 U/ml for 15 minutes at 30 µg

Anti-BRCA1 (phospho S1457) antibody - ab3545 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-BRCA1 (phospho S1466) antibody - ab3614 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-BRCA1 (phospho S1466) antibody (ab3614) at 1/2000 dilutionLane 1 : 25ug HeLa cell extractLane 2 : 25ug HeLa cell lysate, cells treated with 10Gy IR
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