Human/Mouse/Rat HIF-1 alpha Affinity Purified Polyclonal Ab, Goat IgG

• Supplier: R&D Systems
• Catalog: AF1935-SP
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: Simple Western WB IP ChIP
• Species Reactivities: Human, Mouse, Rat
• Antigen: E. coli-derived recombinant human HIF-1 alpha . Arg575-Asn826 Accession Number Q16665.1
• Description: Goat Polyclonal
• Gene: HIF1A
• Conjugate: Unconjugated

Product images

Detection of Human HIF‑1 alpha by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF‑1 alpha Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF‑1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of HIF‑1 alpha -regulated Genes by Chromatin Immunoprecipitation. Mouse primary kidney cells treated with 150 µM CoCl2 for overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF‑1 alpha/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat HIF‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The epo promoter was detected by standard PCR.

Detection of Human HIF‑1 alpha by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF‑1 alpha at approximately 115 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HIF‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Product References

The hypoxic microenvironment upgrades stem-like properties of ovarian cancer - The hypoxic microenvironment upgrades stem-like properties of ovarian cancer

Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z. BMC Cancer. 2012 May 29;12:201.

Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible - Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible

Palladino MA, Shah A, Tyson R, Horvath J, Dugan C, Karpodinis M. Reprod Biol Endocrinol. 2012 Dec 5;10:104.

Development of an in vitro model of myotube ischemia. - Development of an in vitro model of myotube ischemia.

Joshi D, Patel H, Baker DM, Shiwen X, Abraham DJ, Tsui JC. Lab Invest. 2011 Aug;91(8):1241-52.

HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival - HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival

Robador PA, San Jose G, Rodriguez C, Guadall A, Moreno MU, Beaumont J, Fortuno A, Diez J, Martinez-Gonzalez J, Zalba G. Cardiovasc Res. 2011 Nov 1;92(2):247-55.

HER-2 signaling, acquisition of growth factor independence, and regulation of - HER-2 signaling, acquisition of growth factor independence, and regulation of

Bollig-Fischer A, Dziubinski M, Boyer A, Haddad R, Giroux CN, Ethier SP. Cancer Res. 2010 Oct 15;70(20):7862-73.

Hypoxia induces leptin gene expression and secretion in human preadipocytes: - Hypoxia induces leptin gene expression and secretion in human preadipocytes:

Wang B, Wood IS, Trayhurn P. J Endocrinol. 2008 Jul;198(1):127-34.

The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) - The new tumor-suppressor gene inhibitor of growth family member 4 (ING4)

Colla S, Tagliaferri S, Morandi F, Lunghi P, Donofrio G, Martorana D, Mancini C, Lazzaretti M, Mazzera L, Ravanetti L, Bonomini S, Ferrari L, Miranda C, Ladetto M, Neri TM, Neri A, Greco A, Mangoni M, Bonati A, Rizzoli V, Giuliani N. Blood. 2007 Dec 15;110(13):4464-75. Epub 2007 Sep 11.