HIF1A

Gene Symbol HIF1A
Entrez Gene 3091
Alt Symbol HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Species Human
Gene Type protein-coding
Description hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Other Description ARNT interacting protein|ARNT-interacting protein|HIF-1-alpha|PAS domain-containing protein 8|basic-helix-loop-helix-PAS protein MOP1|class E basic helix-loop-helix protein 78|hypoxia-inducible factor 1 alpha isoform I.3|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|hypoxia-inducible factor 1-alpha|hypoxia-inducible factor1alpha|member of PAS protein 1|member of PAS superfamily 1
Swissprots Q16665 Q53XP6 C0LZJ3 Q9UPB1 Q96PT9
Accessions AAC68568 AAF20149 EAW80806 EAW80807 EAW80808 EAW80809 Q16665 AB073325 BAB70608 AB500182 BAI49183 AB733094 BAM28632 AF207601 AAF20139 AF207602 AAF20140 AF304431 AAG43026 AJ227916 AK093749 AK296876 BAG59438 AK299551 BAG61496 AK304315 BAG65167 AK304436 BAG65259 AK312397 BAG35314 AU123241 BC012527 AAH12527 BT009776 AAP88778 BX648795 CN264295 CN264320 DC344032 DQ975378 FJ790247 ACN88547 U22431 AAC50152 U29165 AAC51210 X72726 NM_001243084 NP_001230013 NM_001530 NP_001521 NM_181054 NP_851397
Function Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. {ECO:0000269|PubMed:11292861, EC
Subcellular Location Cytoplasm. Nucleus. Nucleus speckle {ECO:0000250|UniProtKB:Q61221}. Note=Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia. {ECO:0000250|UniProtKB:Q61221}.
Tissue Specificity Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland. {ECO:0000269|PubMed:22009797}.
Top Pathways Renal cell carcinoma, Central carbon metabolism in cancer, Choline metabolism in cancer, Proteoglycans in cancer, mTOR signaling pathway

Hydroxy-HIF-1α (Pro564) (D43B5) XP ® Rabbit mAb - 3434 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D43B5
Western blot analysis of extracts from HeLa cells, treated with either 10 μM of MG132 (to accumulate hydroxylated HIF-1α) or 10 µM MG132 and 1 mM DMOG (to accumulate nonhyroxylated HIF-1α), using Hydroxy-HIF-1α (Pro564) (D43B5) XP ® Rabbit mAb (upper) or total HIF-1α Antibody #3716 (lower).

HIF-1α (D2U3T) Rabbit mAb - 14179 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2U3T
Western blot analysis of extracts from various cell lines, untreated (-) or treated with either DMOG (1 mM, 6 hr; +) or cobalt chloride (100 µM, 4 hr; +) as indicated, using HIF-1α (D2U3T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

HIF-1α Antibody - 3716 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Raji and Jurkat cells, untreated or treated with cobalt chloride (0.1 mM for 4 hours), using HIF-1α Antibody.

HIF-1α (28b) - sc-13515 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HIF-1α (28b): sc-13515. Western blot analysis of HIF-1α expression in extracts prepared from control (A) and CoCl<sub>2</sub>-treated (B) HeLa cultures.

HIF-1α (H-206) - sc-10790 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HIF-1α (H-206): sc-10790. Western blot analysis of HIF-1α expression in K-562 whole cell lysate.

HIF-1α (C-19) - sc-8711 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HIF-1α (H1alpha 67) - sc-53546 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HIF-1α (H1alpha 67): sc-53546. Western blot analysis of HIF-1α expression in CoCl2 treated HeLa immunopre-cipitated with HIF-1α (H1alpha 67) sc-53546 and detected with HIF-1α (H-206) sc-10790.

HIF-1α (Y-15) - sc-12542 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HIF-1α (Y-15): sc-12542. Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic localization.

HIF-1α (3C144) - sc-71247 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-HIF-1-alpha (hydroxy P402) antibody - ab72775 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab72775 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72775, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HIF-1-alpha (hydroxy P564) antibody - ab72777 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HIF-1-alpha antibody - ab85886 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab85886 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85886, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HIF-1-alpha antibody - ab82832 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of HIF-1-alpha staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82832, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-HIF-1-alpha antibody - ab103063 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab103063 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103063 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HIF-1-alpha antibody [1A3] - ab113642 from Abcam

Prices $397.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A3
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling HIF1 alpha (green) using ab113642 at 1/200. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments labeled with Alexa Fluor® 555 phalloidin.

Anti-HIF-1-alpha antibody [EP1215Y] - ab51608 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1215Y
ab51608 staining HIF-1-alpha in HeLa cell line treated with Cocl2 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500). An Alexa Fluor®488-conjugated Goat anti-rabbit IgG(1/200) was used as the secondary antibody. Nuclei were counterstained with DAPI(right hand image).

Anti-HIF-1-alpha antibody [EP1215Y] (Alexa Fluor® 488) - ab190197 from Abcam

Prices $439.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1215Y
ab190197 staining HIF-1α in DFO-treated HeLa cells. The cells were treated with 1mM Desferrioxamine (DFO) for 24 hours or solvent-only for control purposes. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab190197 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at a diltuion of 1 in 250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-HIF-1-alpha antibody [EPR16897] - ab179483 from Abcam

Prices $382.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16897
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HIF-1-alpha with ab179483 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on HeLa cell line. The expression of HIF-1-alpha was increased upon CoCl2 treatment (0.6nM for 6 hours); and the protein also translocated from the cytoplasm into the nucleus. [Biochimica et Biophysica Acta 1745 (2005) 187 – 195.] The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:1. ab179483 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dil

Anti-HIF-1-alpha antibody [H1alpha67] - ab80544 from Abcam

Prices $372.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone H1alpha67

Anti-HIF-1-alpha antibody [HIF1A-84] - ab199004 from Abcam

Prices $359.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone HIF1A-84

Anti-HIF-1-alpha antibody [Hif28b] - ab103409 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone Hif28b

Anti-HIF-1-alpha antibody (HRP) - ab81634 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HIF-1-alpha antibody [OZ12] - ab1817 from Abcam

Prices $370.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone OZ12

Anti-HIF-1-alpha antibody [OZ12] - ab80521 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone OZ12

HIF-1-alpha antibody panel - ab10625 from Abcam

Prices $335.00
Sizes 1 kit
Search more