Anti-c-Kit antibody [2B8] (Biotin)

• Supplier: Abcam
• Catalog: ab25022
• Prices: $388.00
• Sizes: 100 µg
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 2B8
• Applications: IHC-F FC FA ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat
• Antigen: The details of the immunogen for this antibody are not available
• Description: Rat Monoclonal
• Gene: Kit
• Conjugate: Biotin

Product images

Immunostaining of mouse embryonic stem cells using c-Kit (Biotin) ab25022. The antibody bound to the cells was detected by treatment with Cy3-conjugated streptavidin (25 µg/ml). The image shows a fragment of an adherent colony of mouse embryonic stem cell-derived hepatocyte precursor cells stained with the ab25022 antibody and subsequently treated with Cy3-conjugated streptavidin. Cell nuclei were stained with DAPI. The Cy3 fluorescence pattern suggests the predominant expression of c-Kit in actively proliferating cells in the middle of colonies. Please see abreview for ICC/IF protocol details.See Abreview

ab25022 staining c-Kit (red) in murine testis tissue sections by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and blocked with 2% BSA for 20 minutes at 20°C. Samples were incubated with primary antibody (1/100 in 2% BSA + 0.005% Triton X-100 in PBS) for 16 hours at 4°C. Avidin-FITC was used for detection. Nuclei were stained with DAPI (blue).See Abreview

Product References

Reduction of Na/K-ATPase affects cardiac remodeling and increases c-kit cell - Reduction of Na/K-ATPase affects cardiac remodeling and increases c-kit cell

Drummond CA, Sayed M, Evans KL, Shi H, Wang X, Haller ST, Liu J, Cooper CJ, Xie Z, Shapiro JI, Tian J. Am J Physiol Heart Circ Physiol. 2014 Jun 15;306(12):H1631-43. doi:

Scalable selection of hepatocyte- and hepatocyte precursor-like cells from - Scalable selection of hepatocyte- and hepatocyte precursor-like cells from

Drobinskaya I, Linn T, Saric T, Bretzel RG, Bohlen H, Hescheler J, Kolossov E. Stem Cells. 2008 Sep;26(9):2245-56.