Kit

Gene Symbol	Kit
Entrez Gene	16590
Alt Symbol	Bs, CD117, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, SCO1, SCO5, SOW3, Ssm, Tr-kit, W, c-KIT
Species	Mouse
Gene Type	protein-coding
Description	kit oncogene
Other Description	Dominant white spotting\|SCFR\|Steel Factor Receptor\|belly-spot\|c-kit proto-oncogene protein\|dominant spotting\|mast/stem cell growth factor receptor Kit\|proto-oncogene c-Kit\|proto-oncogene tyrosine-protein kinase Kit\|spotted sterile male\|tyrosine-protein kinase Kit
Swissprots	Q6QJB8 Q6QJB7 Q6LEE9 Q61415 Q61417 Q8C8K9 Q61416 P05532 Q7TS86
Accessions	AAA37420 AAL37551 ACT22524 CAA46799 CAA46800 CAJ01232 CAJ15131 EDL37886 P05532 AK046795 BAC32872 AK047010 AK047175 AK145462 BAE26452 AW741410 AY536430 AAS45606 AY536431 AAS45607 BC026713 BC052457 AAH52457 BC075716 AAH75716 BY017579 BY123520 EF472963 ABP63541 X65997 CAA46798 Y00864 CAA68772 NM_001122733 NP_001116205 NM_021099 NP_066922
Function	Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT p
Subcellular Location	Isoform 3: Cytoplasm. Note=Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head. {ECO:0000250}.
Tissue Specificity	Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells. {ECO:0000269\|PubMed:1378413, ECO:0000269\|PubMed:18447649, ECO:0000269\|PubMed:7527401}.
Top Pathways	Rap1 signaling pathway, Cytokine-cytokine receptor interaction, Central carbon metabolism in cancer, PI3K-Akt signaling pathway, Endocytosis

c-Kit (2B8) - sc-19619 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rat
Clonality	Monoclonal

c-Kit (2B8) FITC: sc-19619 FITC. FCM analysis of mouse bone marrow cells. Black line histogram represents the isotype control, normal rat IgG<sub>2b</sub>: sc-2873.

c-Kit (3C11) - sc-53147 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rat
Clonality	Monoclonal

c-Kit (3H1825) - sc-70455 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rat
Clonality	Monoclonal

Anti-c-Kit antibody [2B8] - ab65525 from Abcam

Prices	$370.00
Sizes	250 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

Anti-c-Kit antibody [2B8] (Allophycocyanin) - ab25332 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

Anti-c-Kit antibody [2B8] (Allophycocyanin/Cy5.5 ® ) - ab46790 from Abcam

Prices	$378.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

FACS analysis of BALB/c bone marrow cells double-stained with rat anti-mouse CD117-R-PE (ab46790) at 0.03 µg/106 cells and rat anti-mouse Ly-6G-FITC.

Anti-c-Kit antibody [2B8] (Allophycocyanin/Cy7 ®) - ab79103 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

Anti-c-Kit antibody [2B8] (Biotin) - ab25022 from Abcam

Prices	$388.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

Immunostaining of mouse embryonic stem cells using c-Kit (Biotin) ab25022. The antibody bound to the cells was detected by treatment with Cy3-conjugated streptavidin (25 µg/ml). The image shows a fragment of an adherent colony of mouse embryonic stem cell-derived hepatocyte precursor cells stained with the ab25022 antibody and subsequently treated with Cy3-conjugated streptavidin. Cell nuclei were stained with DAPI. The Cy3 fluorescence pattern suggests the predominant expression of c-Kit in actively proliferating cells in the middle of colonies. Please see abreview for ICC/IF protocol details.See Abreview

Anti-c-Kit antibody [2B8] (Cy5 ®) - ab24984 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

Anti-c-Kit antibody [2B8] (FITC) - ab24870 from Abcam

Prices	$189.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

ab24870 staining cKit in mouse bone marrow cells by Flow Cytometry. 10 µL primary antibody was added to 50 µL cell suspension in PBS with 1% normal mouse serum (500,000 cells). Gating strategy: mesenchymal stem cells.See Abreview

Anti-c-Kit antibody [2B8] (PE/Cy5®) - ab25495 from Abcam

Prices	$186.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

Anti-c-Kit antibody [2B8] (PE/Cy7 ®) - ab95676 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

Flow cytomtry staining of C57BL/6 bone marrow cells with FITC conjugated anti-Human/Mouse CD45R (B220) and (1) PE-Cy7 conjugated Rat IgG2b ? Isotype Control at 0.5 µg or (2) ab95676 at 0.5 µg. Total viable cells were used for analysis.

Anti-c-Kit antibody [2B8] (Phycoerythrin) - ab25364 from Abcam

Prices	$372.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	2B8

Anti-c-Kit antibody [ACK4] - ab112177 from Abcam

Prices	$376.00
Sizes	250 µg
Host	Rat
Clonality	Monoclonal
Clone	ACK4

ab112177 at 0.2µg/106 cells staining c-Kit in Mouse Bone marrow cells (BALB/c strain) by Flow Cytometry. Percentage of cells stained above control (Purified Rat IgG2a ): 7.3%.

Anti-c-Kit antibody [ACK4] (Biotin) - ab112167 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	ACK4

Cell Source: Bone Marrow Percentage of cells stained above control: 9.7%, Using ab112167 at 0.2 µg/106

CD117/c-kit Antibody [Unconjugated] - AF1356 from Novus Biologicals

Prices	$99.00, $369.00
Sizes	25 µg, 100 µg
Host	Goat
Clonality	Polyclonal

Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CD117/c-kit Antibody (180627) - MAB1356 from Novus Biologicals

Prices	$99.00, $269.00, $699.00
Sizes	25 µg, 100 µg, 500 µg
Host	Rat
Clonality	Monoclonal
Clone	180627

CD117/c-kit was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # MAB1356) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to Bergmann glial cells located around Purkinje cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

CD117/c-kit Antibody (9D2) - NB110-93600 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	9D2

Immunohistochemistry: CD117/c-kit Antibody (9D2) [NB110-93600] - Staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals.

CD117/c-kit Antibody (ACK2) - NBP2-22216 from Novus Biologicals

Prices	$369.00
Sizes	500 µg
Host	Rat
Clonality	Monoclonal
Clone	ACK2

CD117/c-kit Antibody (180627) [Biotin] - BAM1356 from Novus Biologicals

Prices	$389.00
Sizes	250 µg
Host	Rat
Clonality	Monoclonal
Clone	180627

CD117/c-kit Antibody [Biotin] - BAF1356 from Novus Biologicals

Prices	$390.00
Sizes	50 µg
Host	Goat
Clonality	Polyclonal

CD117/c-kit Antibody (180627) [Phycoerythrin] - FAB1356P from Novus Biologicals

Prices	$159.00
Sizes	100 tests
Host	Rat
Clonality	Monoclonal
Clone	180627

CD117/c-kit Antibody (180627) [Allophycocyanin] - FAB1356A from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	180627

CD117/c-kit Antibody (ACK2) [Allophycocyanin] - NBP1-43020 from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	ACK2

Flow Cytometry: c-Kit Antibody (ACK2) [Allophycocyanin] [NBP1-43020] - Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b kappa Isotype Control APC (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) APC (right). Total viable cells were used for analysis.

CD117/c-kit Antibody (ACK2) [PE/Cy5] - NBP1-43079 from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	ACK2

Flow Cytometry: c-Kit Antibody (ACK2) [PE/Cy5] [NBP1-43079] - Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype Control PE-Cy5 (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) PE-Cy5 (right). Total viable cells were used for analysis.

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