Anti-CACNA1S antibody [1A]

Name	Anti-CACNA1S antibody [1A]
Supplier	Abcam
Catalog	ab2862
Prices	$370.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	1A
Applications	ICC/IF ICC/IF IHC-F IP WB IHC-P B/N ELISA FC
Species Reactivities	Mouse, Rat, Rabbit, Guinea Pig, Human
Antigen	Full length native protein (purified) corresponding to Rabbit CACNA1S
Description	Mouse Monoclonal
Gene	CACNA1S
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human skeletal muscle tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then incubated with ab2862 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

$Image from Xu X et al, PLoS One. 2010 Apr 13;5(4):e10140, Fig 8.Lanes 1-4 are ab2862 used to detect CACNA1S in lysate prepared from rabbit hearts. Each lane represents a separate heart.Frozen tissue chunks were pulverized under liquid nitrogen, and homogenized in 10 vol of buffer (0.25 M sucrose, 1 mM EDTA [pH 7.4]). This and all the following procedures took place in the presence of the protease inhibitor cocktail at 4°C or on ice. The homogenate was centrifuged at 3,500 rpm for 10 min to pellet nuclei and debris. The supernatant was centrifuged at 30,000 rpm for 1 hr to pellet the membranes. The membrane pellet was washed with a solution (Tris-HCl 20 mM [pH 7.4], EDTA 1 mM) and referred to as post-nuclear membrane fraction. The post-nuclear membrane fraction was rehomogenized in a Triton-containing lysis buffer (Tris-HCl 20 mM [pH 7.5], NaCl 0.2 M, EDTA 1 mM, Triton X-100 1%) using Dounce grinder. The mixture was centrifuged at 17,000 rpm for 1 hr, and the supernatant (Triton extract$ Image from Xu X et al, PLoS One. 2010 Apr 13;5(4):e10140, Fig 8.Lanes 1-4 are ab2862 used to detect CACNA1S in lysate prepared from rabbit hearts. Each lane represents a separate heart.Frozen tissue chunks were pulverized under liquid nitrogen, and homogenized in 10 vol of buffer (0.25 M sucrose, 1 mM EDTA [pH 7.4]). This and all the following procedures took place in the presence of the protease inhibitor cocktail at 4°C or on ice. The homogenate was centrifuged at 3,500 rpm for 10 min to pellet nuclei and debris. The supernatant was centrifuged at 30,000 rpm for 1 hr to pellet the membranes. The membrane pellet was washed with a solution (Tris-HCl 20 mM [pH 7.4], EDTA 1 mM) and referred to as post-nuclear membrane fraction. The post-nuclear membrane fraction was rehomogenized in a Triton-containing lysis buffer (Tris-HCl 20 mM [pH 7.5], NaCl 0.2 M, EDTA 1 mM, Triton X-100 1%) using Dounce grinder. The mixture was centrifuged at 17,000 rpm for 1 hr, and the supernatant (Triton extract

Product References

Chromatin remodeling as a mechanism for circadian prolactin transcription: - Chromatin remodeling as a mechanism for circadian prolactin transcription:

Guillaumond F, Boyer B, Becquet D, Guillen S, Kuhn L, Garin J, Belghazi M, Bosler O, Franc JL, Francois-Bellan AM. FASEB J. 2011 Aug;25(8):2740-56.

Upregulation of the CaV 1.1-ryanodine receptor complex in a rat model of critical - Upregulation of the CaV 1.1-ryanodine receptor complex in a rat model of critical

Kraner SD, Wang Q, Novak KR, Cheng D, Cool DR, Peng J, Rich MM. Am J Physiol Regul Integr Comp Physiol. 2011 Jun;300(6):R1384-91. doi:

Estimating the risk of drug-induced proarrhythmia using human induced pluripotent - Estimating the risk of drug-induced proarrhythmia using human induced pluripotent

Guo L, Abrams RM, Babiarz JE, Cohen JD, Kameoka S, Sanders MJ, Chiao E, Kolaja KL. Toxicol Sci. 2011 Sep;123(1):281-9.

Long-term fish oil supplementation induces cardiac electrical remodeling by - Long-term fish oil supplementation induces cardiac electrical remodeling by

Xu X, Jiang M, Wang Y, Smith T, Baumgarten CM, Wood MA, Tseng GN. PLoS One. 2010 Apr 13;5(4):e10140.

Cardiomyocyte clusters derived from human embryonic stem cells share similarities - Cardiomyocyte clusters derived from human embryonic stem cells share similarities

Asp J, Steel D, Jonsson M, Ameen C, Dahlenborg K, Jeppsson A, Lindahl A, Sartipy P. J Mol Cell Biol. 2010 Oct;2(5):276-83.

The beta 1a subunit is essential for the assembly of dihydropyridine-receptor - The beta 1a subunit is essential for the assembly of dihydropyridine-receptor

Schredelseker J, Di Biase V, Obermair GJ, Felder ET, Flucher BE, Franzini-Armstrong C, Grabner M. Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17219-24. Epub 2005 Nov 14.

Monoclonal antibody identifies a 200-kDa subunit of the dihydropyridine-sensitive - Monoclonal antibody identifies a 200-kDa subunit of the dihydropyridine-sensitive

Morton ME, Froehner SC. J Biol Chem. 1987 Sep 5;262(25):11904-7.