Anti-Calpain 1 antibody
| Name | Anti-Calpain 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39170 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide based on Domain IV of the large subunit of Human Calpain 1 |
| Description | Rabbit Polyclonal |
| Gene | CAPN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab39170 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Overexpression of the calpain-specific inhibitor calpastatin reduces human - Overexpression of the calpain-specific inhibitor calpastatin reduces human
Diepenbroek M, Casadei N, Esmer H, Saido TC, Takano J, Kahle PJ, Nixon RA, Rao MV, Melki R, Pieri L, Helling S, Marcus K, Krueger R, Masliah E, Riess O, Nuber S. Hum Mol Genet. 2014 Aug 1;23(15):3975-89.
Calpain-2 compensation promotes angiotensin II-induced ascending and abdominal - Calpain-2 compensation promotes angiotensin II-induced ascending and abdominal
Subramanian V, Moorleghen JJ, Balakrishnan A, Howatt DA, Chishti AH, Uchida HA. PLoS One. 2013 Aug 19;8(8):e72214.
Redox control of the senescence regulator interleukin-1alpha and the secretory - Redox control of the senescence regulator interleukin-1alpha and the secretory
McCarthy DA, Clark RR, Bartling TR, Trebak M, Melendez JA. J Biol Chem. 2013 Nov 8;288(45):32149-59.
Calpains mediate epithelial-cell death during mammary gland involution: - Calpains mediate epithelial-cell death during mammary gland involution:
Arnandis T, Ferrer-Vicens I, Garcia-Trevijano ER, Miralles VJ, Garcia C, Torres L, Vina JR, Zaragoza R. Cell Death Differ. 2012 Sep;19(9):1536-48.
Calpain-mediated ataxin-3 cleavage in the molecular pathogenesis of - Calpain-mediated ataxin-3 cleavage in the molecular pathogenesis of
Hubener J, Weber JJ, Richter C, Honold L, Weiss A, Murad F, Breuer P, Wullner U, Bellstedt P, Paquet-Durand F, Takano J, Saido TC, Riess O, Nguyen HP. Hum Mol Genet. 2013 Feb 1;22(3):508-18.
Estrogen regulates cytoskeletal flexibility, cellular metabolism and synaptic - Estrogen regulates cytoskeletal flexibility, cellular metabolism and synaptic
Szego EM, Kekesi KA, Szabo Z, Janaky T, Juhasz GD. Psychoneuroendocrinology. 2010 Jul;35(6):807-19. doi:
Calpain expression and activity during lens fiber cell differentiation. - Calpain expression and activity during lens fiber cell differentiation.
De Maria A, Shi Y, Kumar NM, Bassnett S. J Biol Chem. 2009 May 15;284(20):13542-50.
Asymmetric localization of calpain 2 during neutrophil chemotaxis. - Asymmetric localization of calpain 2 during neutrophil chemotaxis.
Nuzzi PA, Senetar MA, Huttenlocher A. Mol Biol Cell. 2007 Mar;18(3):795-805. Epub 2006 Dec 27.