CAPN1

Gene Symbol CAPN1
Entrez Gene 823
Alt Symbol CANP, CANP1, CANPL1, muCANP, muCL
Species Human
Gene Type protein-coding
Description calpain 1, (mu/I) large subunit
Other Description CANP 1|calcium-activated neutral proteinase 1|calpain mu-type|calpain, large polypeptide L1|calpain-1 catalytic subunit|calpain-1 large subunit|cell proliferation-inducing gene 30 protein|cell proliferation-inducing protein 30|micromolar-calpain
Swissprots Q2TTR0 Q6DHV4 P07384
Accessions AAV41878 EAW74366 EAW74367 EAW74368 EAW74369 EAW74370 EAW74371 P07384 AK022319 AK025380 AK097277 AK301538 BAG63037 AK311001 AK315556 BAG37932 AY550975 AAT52221 BC008751 AAH08751 BC015091 BC017200 AAH17200 BC075862 AAH75862 BE018156 DA788342 DC365116 DC372552 DQ892905 ABM83831 DQ896600 ABM87599 X04366 CAA27881 XM_006718698 XP_006718761 XM_011545292 XP_011543594 NM_001198868 NP_001185797 NM_001198869 NP_001185798 NM_005186 NP_005177 NR_040008
Function Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:8769305}. Cell membrane {ECO:0000269|PubMed:8769305}. Note=Translocates to the plasma membrane upon Ca(2+) binding.
Tissue Specificity Ubiquitous.
Top Pathways Apoptosis, Alzheimer's disease, Protein processing in endoplasmic reticulum

Calpain 1 Large Subunit (Mu-type) Antibody - 2556 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat and U937 cells, using Calpain 1 Large Subunit (Mu-type) Antibody.

Calpain 1 (A-5) - sc-390677 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calpain 1 (A-5): sc-390677. Western blot analysis of Calpain 1 expression in A431 (A), K-562 (B) and PC-3 (C) whole cell lysates.

Calpain 1 (D-11) - sc-271313 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calpain 1 (D-11): sc-271313. Western blot analysis of Calpain expression in K-562 whole cell lysate.

Calpain 1 (C-20) - sc-7530 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Calpain 1 (C-20): sc-7530. Western blot analysis of recombinant human erythrocyte Calpain 1 (A) and Calpain 1 expression in TF-1 (B), K-562 (C) and A-431 (D) whole cell lysates.

Calpain 1 (6C-12) - sc-32327 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calpain 1 (6C-12): sc-32327. Western blot analysis of Calpain 1 expression in human PBL (A), TF-1 (B) and K-562 (C) whole cell lysates.

Calpain 1 (K-17) - sc-31065 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Calpain 1 (P-6) - sc-81171 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calpain 1 (P-6): sc-81171. Western blot analysis of Calpain 1 expression in A-431 (A), TF-1 (B) and K-562 (C) whole cell lysates.

Calpain 1 (L-18) - sc-31066 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Calpain 1 (L-18): sc-31066. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.

Calpain 1 (N-19) - sc-7531 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Calpain 1 (N-19): sc-7531. Western blot analysis of human recombinant Calpain 1.

Calpain 1 (H-65) - sc-13990 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Calpain 1 (H-65): sc-13990. Western blot analysis of Calpain 1 expression in HeLa whole cell lysate.

Calpain 1 (5B3) - sc-32796 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calpain 1 (5B3): sc-32796. Western blot analysis of Calpain 1 small subunit expression in K-562 (A) and A-431 (B) whole cell lysates.

Calpain 1/2 (28F3) - sc-58326 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Calpain 1/2 (28F3): sc-58326. Western blot analysis of Calpain 2 expression in normal human fibroblast (A) and Madin-Darby bovine kidney (B) whole cell lysates.

Anti-Calpain 1 antibody - ab28258 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Calpain 1 antibody (ab28258) at 1/1000 dilution + Calpain 1 at 10 µg

Anti-Calpain 1 antibody - ab39170 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab39170 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Calpain 1 antibody - ab28257 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab28257 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28257, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Calpain 1 antibody - ab39171 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab39171 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39171, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Calpain 1 antibody - ab155677 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Calpain 1 antibody (ab155677) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

Anti-Calpain 1 antibody - ab89778 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Calpain 1 antibody (ab89778) at 1 µg/ml + human liver tissue lysate at 50 µg

Anti-Calpain 1 antibody - ab2543 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Calpain 1 antibody (ab2543) at 1 µg/ml + Active human Calpain 1 full length protein (ab91019) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds

Anti-Calpain 1 antibody [EPR3319] - ab108400 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3319
Overlay histogram showing Jurkat cells stained with ab108400 (red line) at 1/380 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

Anti-Calpain 1 antibody [EPR3323] - ab92356 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3323
All lanes : Anti-Calpain 1 antibody [EPR3323] (ab92356) at 1/1000 dilutionLane 1 : T47-D cell lysateLane 2 : 293T cell lysateLane 3 : A431 cell lysateLane 4 : Human spleen lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit antibody at 1/2000 dilution

Anti-Calpain 1 antibody [SP81] - ab101683 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP81
Immunohistochemical analysis of Calpain expression in formalin-fixed, paraffin-embedded Human breast carcinoma using ab101683 at 1/100 dilution.

Anti-CAPN1 / Calpain 1 Antibody (aa515-526) IHC-plus™ - LS-B11190 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-CAPN1 / Calpain 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11190 concentration 5 ug/ml.

Anti-CAPN1 / Calpain 1 Antibody (clone 1376CT809.24.85) - LS-C344156 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 1376CT809.24.85
Immunohistochemical of paraffin-embedded H. kidney section using CAPN1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Anti-CAPN1 / Calpain 1 Antibody (aa468-481) IHC-plus™ - LS-B11191 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-CAPN1 / Calpain 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11191 concentration 5 ug/ml.
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