Anti-Calpain 1 antibody
| Name | Anti-Calpain 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28257 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide based on the amino terminal end of the large subunit of human calpain 1 |
| Description | Rabbit Polyclonal |
| Gene | CAPN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab28257 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28257, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Calpain 1 antibody (ab28257) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Redox control of the senescence regulator interleukin-1alpha and the secretory - Redox control of the senescence regulator interleukin-1alpha and the secretory
McCarthy DA, Clark RR, Bartling TR, Trebak M, Melendez JA. J Biol Chem. 2013 Nov 8;288(45):32149-59.
Calpains mediate epithelial-cell death during mammary gland involution: - Calpains mediate epithelial-cell death during mammary gland involution:
Arnandis T, Ferrer-Vicens I, Garcia-Trevijano ER, Miralles VJ, Garcia C, Torres L, Vina JR, Zaragoza R. Cell Death Differ. 2012 Sep;19(9):1536-48.
Calpain-1 cleaves Rad21 to promote sister chromatid separation. - Calpain-1 cleaves Rad21 to promote sister chromatid separation.
Panigrahi AK, Zhang N, Mao Q, Pati D. Mol Cell Biol. 2011 Nov;31(21):4335-47.
Calpain 1 and Calpastatin expression is developmentally regulated in rat brain. - Calpain 1 and Calpastatin expression is developmentally regulated in rat brain.
Li Y, Bondada V, Joshi A, Geddes JW. Exp Neurol. 2009 Dec;220(2):316-9.
N terminus of calpain 1 is a mitochondrial targeting sequence. - N terminus of calpain 1 is a mitochondrial targeting sequence.
Badugu R, Garcia M, Bondada V, Joshi A, Geddes JW. J Biol Chem. 2008 Feb 8;283(6):3409-17. Epub 2007 Dec 10.