Anti-Calpain 1 antibody [EPR3319]

• Supplier: Abcam
• Catalog: ab108400
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3319
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues in Human Calpain
• Description: Rabbit Monoclonal
• Gene: CAPN1
• Conjugate: Unconjugated

Product images

Overlay histogram showing Jurkat cells stained with ab108400 (red line) at 1/380 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

ab108400 staining Calpain 1 in the T-47D cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/70). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab108400 staining Calpain 1 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/200). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution + C6 Lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/5000 dilution + T47-D Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/5000 dilution + Human fetal kidney lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution (unpurified)Lane 1 : Human fetal kidney cell lysateLane 2 : U937 cell lysateLane 3 : A431 cell lysateLane 4 : 293T cell lysateLane 5 : T47D cell lysateLysates/proteins at 10 µg per lane.

ab108400, unpurified, at 1/500 dilution staining Calpain in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.

ab108400, unpurified, at 1/250 dilution staining Calpain in HeLa cells by Immunofluorescence.

Product References

The role of calpain-myosin 9-Rab7b pathway in mediating the expression of - The role of calpain-myosin 9-Rab7b pathway in mediating the expression of

Tsai JC, Lin YW, Huang CY, Lin CY, Tsai YT, Shih CM, Lee CY, Chen YH, Li CY, Chang NC, Lin FY, Tsai CS. PLoS One. 2014 Jan 28;9(1):e85833.

How does colistin-induced nephropathy develop and can it be treated? - How does colistin-induced nephropathy develop and can it be treated?

Ozkan G, Ulusoy S, Orem A, Alkanat M, Mungan S, Yulug E, Yucesan FB. Antimicrob Agents Chemother. 2013 Aug;57(8):3463-9.