Anti-CaMKII alpha (phospho T286) antibody [22B1]
| Name | Anti-CaMKII alpha (phospho T286) antibody [22B1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2724 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 22B1 |
| Applications | FC ICC/IF ICC/IF ELISA IP WB |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic peptide: (281)MHRQET(p)VDCLKKFN(294) |
| Description | Mouse Monoclonal |
| Gene | CAMK2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab2724 stained Hep cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2724, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing SH-SY5Y cells stained with ab2724 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2724, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/20006_CaMKII-alpha-Primary-antibodies-ab2724-3.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab2724 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2724, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
CaMKII, but not protein kinase A, regulates Rpt6 phosphorylation and proteasome - CaMKII, but not protein kinase A, regulates Rpt6 phosphorylation and proteasome
Jarome TJ, Kwapis JL, Ruenzel WL, Helmstetter FJ. Front Behav Neurosci. 2013 Aug 30;7:115.
Rapid, bidirectional remodeling of synaptic NMDA receptor subunit composition by - Rapid, bidirectional remodeling of synaptic NMDA receptor subunit composition by
Jung SC, Kim J, Hoffman DA. Neuron. 2008 Nov 26;60(4):657-71.
Comprehensive identification of phosphorylation sites in postsynaptic density - Comprehensive identification of phosphorylation sites in postsynaptic density
Trinidad JC, Specht CG, Thalhammer A, Schoepfer R, Burlingame AL. Mol Cell Proteomics. 2006 May;5(5):914-22. Epub 2006 Feb 1.