![All lanes : Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (ab76238) at 1/200000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/20200_ab76238_WB_3.jpg)
All lanes : Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (ab76238) at 1/200000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/1000 dilution
ab76238, at a 1/100 dilution, staining human cAMP Protein Kinase Catalytic Subunit in testis by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
ICC/IF image of ab76238 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76238, neat) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing HeLa cells stained with ab76238 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76238, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.