PRKACA

Gene Symbol PRKACA
Entrez Gene 5566
Alt Symbol PKACA, PPNAD4
Species Human
Gene Type protein-coding
Description protein kinase, cAMP-dependent, catalytic, alpha
Other Description PKA C-alpha|cAMP-dependent protein kinase catalytic subunit alpha|protein kinase A catalytic subunit
Swissprots Q32P54 Q9NRH9 Q9H2Y0 Q9NRB4 P17612
Accessions ABG25918 EAW84397 EAW84398 EAW84399 EAW84400 P17612 AF208004 AAG35720 AF224718 AAF75622 AF239744 AAF76426 AK093803 AK131561 AK290147 BAF82836 AK292284 BAF84973 AK301264 BAH13444 BC039846 AAH39846 BC108259 AAI08260 BU543573 M80335 AAA60094 X07767 CAA30597 NM_001304349 NP_001291278 NM_002730 NP_002721 NM_207518 NP_997401
Function Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose- mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B- alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasi
Subcellular Location Cytoplasm. Cell membrane. Nucleus {ECO:0000250}. Mitochondrion {ECO:0000250}. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). {ECO:0000250}.
Tissue Specificity Isoform 1 is ubiquitous. Isoform 2 is sperm- specific and is enriched in pachytene spermatocytes but is not detected in round spermatids. {ECO:0000269|PubMed:10906071, ECO:0000269|PubMed:21812984}.
Top Pathways Calcium signaling pathway, Ovarian steroidogenesis, MAPK signaling pathway, Estrogen signaling pathway, Adrenergic signaling in cardiomyocytes

Phospho-PKA C (Thr197) Antibody - 4781 from Cell Signaling Technology

Prices $279.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C6 and NIH/3T3 cells, untreated or treated with lambda phosphatase (PPase), using Phospho-PKA C (Thr197) Antibody (upper) or PKA C-alpha Antibody #4782 (lower).

Phospho-PKA C (Thr197) (D45D3) Rabbit mAb - 5661 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D45D3
Western blot analysis of extracts from NIH/3T3 cells, untreated or λ phosphatase-treated, using Phospho-PKA C (Thr197) (D45D3) Rabbit mAb (upper) or PKA C-α Antibody #4782 (lower).

PKA C-α Antibody - 4782 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, C6, PC12 and NIH/3T3 cells, using PKA C-α Antibody.

PKA C-α (D38C6) Rabbit mAb - 5842 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D38C6
Western blot analysis of extracts from HeLa, C6, and COS-7 cells using PKA C-α (D38C6) Rabbit mAb.

PKAα cat (A-2) - sc-28315 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Monoclonal
PKAα cat (A-2): sc-28315. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplasmic staining of neuronal cells, glial cells and endothelial cells.

PKAα cat (N-18) - sc-30666 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Polyclonal
PKAα cat (N-18): sc-30666. Western blot analysis of PKAα cat expression in mouse brain (A), rat testis (B) and human heart (C) tissue extracts and U-251-MG whole cell lysate (D).

PKAα cat (C-20) - sc-903 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Polyclonal
PKAα cat (C-20): sc-903. Western blot analysis of PKAα catalytic subunit expression in mouse brain extract (A), NIH/3T3 (B), KNRK (C), Mv 1 Lu (D), MDCK (E), MCF7 (F), PC-3 (G) and A549 (H) whole cell lysates.

PKAα cat (C-7) - sc-48412 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Monoclonal
PKAα cat (C-7): sc-48412. Western blot analysis of PKAα cat expression in non-transfected 293T: sc-117752 (A), human PKAα cat transfected 293T: sc-115793 (B) and A549 (C) whole cell lysates.

PKAα cat (W-18) - sc-30669 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Polyclonal

PKAα cat (B-5) - sc-28316 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Monoclonal
PKAα cat (B-5): sc-28316. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in ductus seminiferus and Leydig cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

PKAα/β cat (F-20) - sc-30668 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Polyclonal
PKAα/β cat (F-20): sc-30668. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

PKAα/β/γ cat (B-4) - sc-365615 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Monoclonal
PKAα/β/γ cat (B-4): sc-365615. Western blot analysis of PKAα/β/γ expression in MCF7 (A) and PC-3 (B) whole cell lysates.

PKAα/β/γ cat (G-6) - sc-390548 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Monoclonal
PKAα/β/γ cat (G-6): sc-390548. Western blot analysis of PKAα/β/γ cat expression in NIH/3T3 (A) and PC-3 (B) whole cell lysates and mouse brain (C) and human hippocampus (D) tissue extracts.

PKAα/β/γ cat (H-95) - sc-28892 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Polyclonal
PKAα/β/γ cat (H-95): sc-28892. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

p-PKAα/β/γ cat (Thr 198) - sc-32968 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Cat
Clonality Polyclonal
p-PKAα/β/γ cat (Thr 198): sc-32968. Western blot analysis of PKAα cat phosphorylation in non-transfected: sc-117752 (A) and human PKAα cat transfected: sc-111700 (B) 293T whole cell lysates.

Anti-cAMP Protein Kinase Catalytic subunit antibody - ab59253 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-cAMP Protein Kinase Catalytic subunit antibody (ab59253) at 1/500 dilutionLane 1 : mouse brain cell extractsLane 2 : mouse brain cell extracts with immunising peptide

Anti-cAMP Protein Kinase Catalytic subunit antibody - ab136960 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-cAMP Protein Kinase Catalytic subunit antibody (ab136960) at 1/500 dilution + RAW 264.7 cell lysate at 30 µg

Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - ab76238 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2102Y
All lanes : Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (ab76238) at 1/200000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/1000 dilution

Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody - ab59218 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59218 at 1/50 dilution staining PKA alpha + beta (catalytic subunit) in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence (left) or presence (right) of the immunising peptide.

Anti-PKA alpha + beta (catalytic subunits) antibody - N-terminal - ab71764 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKA alpha + beta (catalytic subunits) antibody - N-terminal (ab71764) at 1/100 dilutionLane 1 : Jurkat cell lysate Lane 2 : mouse heart tissue lysate Lysates/proteins at 12.5 µg per lane.

Anti-PRKACA antibody - ab76513 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-PRKACA antibody (ab76513) at 5 µg/ml + immunogen (GST-tagged recombinant fragment) at 0.2 µg/mlSecondaryGoat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

Anti-PRKACA Antibody (phospho-Ser657/Tyr658) - LS-C75170 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKACA + PRKACB Antibody (aa11) - LS-C190239 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PRKACA Antibody (aa1-121, clone X1) - LS-C309264 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone X1

Anti-PRKACB Antibody (aa291-319) - LS-C330008 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more