Anti-Cathepsin D antibody [4G2]
| Name | Anti-Cathepsin D antibody [4G2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40697 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 4G2 |
| Applications | ELISA WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 21-412 of Human Cathepsin D |
| Description | Mouse Monoclonal |
| Gene | CTSD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing MCF7 cells stained with ab40697 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40697, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21570_ab40697-5-ab40697FC.jpg)
Overlay histogram showing MCF7 cells stained with ab40697 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40697, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-Cathepsin D antibody [4G2] (ab40697) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Hep3B cell lysateLane 3 : MDA-MB-231 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 20 µg per lane.developed using the ECL techniqueObserved band size : 45 kDa (why is the actual band size different from the predicted?)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21571_40697_1.jpg)
All lanes : Anti-Cathepsin D antibody [4G2] (ab40697) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Hep3B cell lysateLane 3 : MDA-MB-231 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 20 µg per lane.developed using the ECL techniqueObserved band size : 45 kDa (why is the actual band size different from the predicted?)
developed using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
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