Anti-Cathepsin D antibody [CTD-19]
| Name | Anti-Cathepsin D antibody [CTD-19] |
|---|---|
| Supplier | Abcam |
| Catalog | ab6313 |
| Prices | $397.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | CTD-19 |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Human |
| Antigen | Full length native protein (purified) (Human liver) |
| Description | Mouse Monoclonal |
| Gene | CTSD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab6313 stained human HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6313, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and MCF7 cells.
![Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21574_ab6313_5.jpg)
Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells
Product References
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