Anti-Caveolin-3 antibody
| Name | Anti-Caveolin-3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2912 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF IHC-F ICC/IF WB IP |
| Species Reactivities | Mouse, Rat, Sheep, Human |
| Antigen | Synthetic peptide corresponding to Mouse Caveolin-3 aa 1-19 |
| Description | Rabbit Polyclonal |
| Gene | CAV3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent analysis of Caveolin-3 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) (right panel) or with ab2912 at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Caveolin-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunofluorescent analysis of Caveolin-3 in C2C11 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) (right panel) or with ab2912 at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Caveolin-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Mouse heart tissue sections stained for caveolin-3. Primary ab used was ab2912 and secondary was an anti-rabbit goat polyclonal antibody conjugated to Cy3.See Abreview
Western blot of caveolin-3 on rat cardiac muscle protein extract using ab2912. Western blot of caveolin-3 on rat cardiac muscle protein extract using ab2912.
Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Caveolin-3 ab2912 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing Caveolin-3 ab2912 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing Caveolin-3 ab2912 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Product References
Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin - Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin
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Chymase mediates injury and mitochondrial damage in cardiomyocytes during acute - Chymase mediates injury and mitochondrial damage in cardiomyocytes during acute
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Annotation of loci from genome-wide association studies using tissue-specific - Annotation of loci from genome-wide association studies using tissue-specific
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Muscle protein synthesis, mTORC1/MAPK/Hippo signaling, and capillary density are - Muscle protein synthesis, mTORC1/MAPK/Hippo signaling, and capillary density are
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Deletion of cavin genes reveals tissue-specific mechanisms for morphogenesis of - Deletion of cavin genes reveals tissue-specific mechanisms for morphogenesis of
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Four weeks of normobaric "live high-train low" do not alter muscular or systemic - Four weeks of normobaric "live high-train low" do not alter muscular or systemic
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Characterization of the molecular architecture of human caveolin-3 and - Characterization of the molecular architecture of human caveolin-3 and
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High throughput screening for compounds that alter muscle cell glycosylation - High throughput screening for compounds that alter muscle cell glycosylation
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Antibody-directed myostatin inhibition enhances muscle mass and function in - Antibody-directed myostatin inhibition enhances muscle mass and function in
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Protein kinase Calpha activity is important for contraction-induced FXYD1 - Protein kinase Calpha activity is important for contraction-induced FXYD1
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