All lanes : Anti-CD11a antibody (ab89725) at 1 µg/mlLane 1 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of CD11a staining in FFPE human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89725, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.