Anti-CD63 antibody
| Name | Anti-CD63 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab118307 |
| Prices | $376.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to a region within C terminal amino acids 152-201 (TDWEKIPSMS KNRVPDSCCI NVTVGCGINF NEKAIHKEGC VEKIGGWLRK) of Human CD63 (NP_001771) |
| Description | Rabbit Polyclonal |
| Gene | CD63 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab118307 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab118307, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Anti-CD63 antibody (ab118307) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg
