Anti-CD64 antibody [10.1]
| Name | Anti-CD64 antibody [10.1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab119843 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 10.1 |
| Applications | WB IP IHC-P IHC-F FA ICC/IF FC B/N |
| Species Reactivities | Human, Primate |
| Antigen | Rheumatoid synovial fluid cells and fibronectin purified Human monocytes |
| Description | Mouse Monoclonal |
| Gene | FCGR1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing THP1 cells stained with ab119843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119843, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25485_CD64-Primary-antibodies-ab119843-1.jpg)
Overlay histogram showing THP1 cells stained with ab119843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119843, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic - CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic
Sanchez-Torres C, Garcia-Romo GS, Cornejo-Cortes MA, Rivas-Carvalho A, Sanchez-Schmitz G. Int Immunol. 2001 Dec;13(12):1571-81.
The Fc receptor, FcRI, and other activation molecules on human mononuclear - The Fc receptor, FcRI, and other activation molecules on human mononuclear
Jayaram Y, Buckle AM, Hogg N. Clin Exp Immunol. 1989 Mar;75(3):414-20.
The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a - The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a
Dougherty GJ, Selvendran Y, Murdoch S, Palmer DG, Hogg N. Eur J Immunol. 1987 Oct;17(10):1453-9.