Anti-Cdc6 antibody - N-terminal (ab188423) at 1/1000 dilution + HeLa cell lysate at 10 µg
Immunoprecipitation analysis of HeLa whole cell lysate.Lane 1: Control immunoprecipitation with non-immune IgGLane 2: Immunoprecipitates wtih ab188423Lane 3: Input. Western blot detection with ab188423 at 1/1000 dilution.
Immunofluorescent analysis of HeLa cells in G1 or early S phase. Cells in mid to late G1phase or hydroxyurea-treated early S phase were fixed with formaldehyde directly or after extraction with Triton X-100 and then immunostained using ab188423 at 1/100 dilution or control non-immune antibody (Alexa;green). The samples were further treated with propidium iodide for DNA staining (PI; red).