Anti-cIAP2 antibody [E40]
| Name | Anti-cIAP2 antibody [E40] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32059 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | E40 |
| Applications | WB IHC-P ICC/IF IP |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to the residues before the breakpoint for translocation to form BIRC3-MALT1 of Human cIAP2 |
| Description | Rabbit Monoclonal |
| Gene | BIRC3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-cIAP2 antibody [E40] (ab32059) at 1/1000 dilution + Daudi cell lysate.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/28873_ab32059_1.jpg)
Anti-cIAP2 antibody [E40] (ab32059) at 1/1000 dilution + Daudi cell lysate.
ab32059 at a 1:50 dilution staining cIAP2 in human normal spleen, using Immunohistochemistry, Paraffin Embedded Tissue.
![cIAP2 was immunoprecipitated using 0.5mg Daudi whole cell extract, 5µg of Rabbit polyclonal to cIAP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Daudi whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32059.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 72kDa; cIAP2](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/28875_cIAP2-Primary-antibodies-ab32059-5.jpg)
cIAP2 was immunoprecipitated using 0.5mg Daudi whole cell extract, 5µg of Rabbit polyclonal to cIAP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Daudi whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32059.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 72kDa; cIAP2
Product References
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