Anti-CKII alpha antibody [EP1963Y]
| Name | Anti-CKII alpha antibody [EP1963Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab76040 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1963Y |
| Applications | WB FC IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues near the N terminus of human CKII alpha protein |
| Blocking Peptide | CKII alpha peptide |
| Description | Rabbit Monoclonal |
| Gene | CSNK2A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing Jurkat cells stained with ab76040 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76040, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-CKII alpha antibody [EP1963Y] (ab76040) at 1/1000 dilution + HeLa cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/29162_ab76040_1.JPG)
Anti-CKII alpha antibody [EP1963Y] (ab76040) at 1/1000 dilution + HeLa cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of CKII alpha in paraffin embedded human breast carcinoma tissue using ab76040 at a 1/50 dilution.