Flow cytometric analysis of HeLa cells (4% paraformaldehyde-fixed; methanol-permeablized) labeling CPT1A with ab171449 at 5 µg/mL (green) compared with an isotype control ab170192 at 5 µg/mL (black). Isotype control was labeled with a Goat Anti-mouse Alexa Fluor® 488 secondary antibody prior to signal measurement on the FL-1 channel.
Immunofluoresecence analysis of HeLa cells (4% paraformaldehyde-fixed, methanol-permeablized) labeling CPT1A with ab171449 at 5µg/ml overnight. 100x magnification.A) HeLa cells labeled with ab171449 at 5 µg/mL.B) HeLa cells labeled with Anti-HSP60 (1/3000, ab46798), Secondary antibody used was goat anti-rabbit Dylight-594 (1/1000, ab96897).C) Merged Image of A and B showing specificity of mitochondrial staining.