Anti-CYP11A1 antibody
| Name | Anti-CYP11A1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab75497 |
| Prices | $392.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP IHC-P WB ELISA |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to a region of Human CYP11A1 |
| Description | Rabbit Polyclonal |
| Gene | CYP11A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry with HK2 cells.
Immunohistochemistry with HK2 cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling CYP11A1 with ab75497 at 5.0ug/ml.
Immunohistochemistry with HK2 cell lysate tissue
Anti-CYP11A1 antibody (ab75497) at 1 µg/ml + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
![CYP11A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to CYP11A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75497.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: Band: 55kDa: CYP11A1.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/4299_CYP11A1-Primary-antibodies-ab75497-4.jpg)
CYP11A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to CYP11A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75497.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: Band: 55kDa: CYP11A1.
ICC/IF image of ab75497 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75497, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab75497 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75497, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
De novo synthesis of steroids and oxysterols in adipocytes. - De novo synthesis of steroids and oxysterols in adipocytes.
Li J, Daly E, Campioli E, Wabitsch M, Papadopoulos V. J Biol Chem. 2014 Jan 10;289(2):747-64.
Steroidogenic enzyme Cyp11a1 regulates Type 2 CD8+ T cell skewing in allergic - Steroidogenic enzyme Cyp11a1 regulates Type 2 CD8+ T cell skewing in allergic
Jia Y, Domenico J, Takeda K, Han J, Wang M, Armstrong M, Reisdorph N, O'Connor BP, Lucas JJ, Gelfand EW. Proc Natl Acad Sci U S A. 2013 May 14;110(20):8152-7. doi:
The steroidogenic enzyme Cyp11a1 is essential for development of peanut-induced - The steroidogenic enzyme Cyp11a1 is essential for development of peanut-induced
Wang M, Ramirez J, Han J, Jia Y, Domenico J, Seibold MA, Hagman JR, Gelfand EW. J Allergy Clin Immunol. 2013 Nov;132(5):1174-1183.e8. doi:
Mitotane exhibits dual effects on steroidogenic enzymes gene transcription under - Mitotane exhibits dual effects on steroidogenic enzymes gene transcription under
Lin CW, Chang YH, Pu HF. Toxicology. 2012 Aug 16;298(1-3):14-23.
Toll-like receptor-initiated testicular innate immune responses in mouse Leydig - Toll-like receptor-initiated testicular innate immune responses in mouse Leydig
Shang T, Zhang X, Wang T, Sun B, Deng T, Han D. Endocrinology. 2011 Jul;152(7):2827-36.