Anti-Dicer antibody
| Name | Anti-Dicer antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5818 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Dicer |
| Description | Rabbit Polyclonal |
| Gene | DICER1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab5818 (1/200) staining Dicer in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.See Abreview
All lanes : Anti-Dicer antibody (ab5818) at 2 µg/mlLane 1 : HeLa Whole Cell Lysate at 10 µgLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µgLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µgLane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µgLane 5 : HEK293 Whole Cell Lysate Overexpressing Dicer at 5 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab5818 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5818, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.