DICER1

Gene Symbol DICER1
Entrez Gene 23405
Alt Symbol DCR1, Dicer, Dicer1e, HERNA, K12H4.8-LIKE, MNG1, RMSE2
Species Human
Gene Type protein-coding
Description dicer 1, ribonuclease type III
Other Description Dicer1, Dcr-1 homolog|dicer 1, double-stranded RNA-specific endoribonuclease|endoribonuclease Dicer|helicase MOI|helicase with RNAse motif
Swissprots E0AD28 Q9UQ02 B3KRG4 A7E2D3 Q9UPY3 O95943
Accessions CBM42912 CBV35645 EAW81595 EAW81596 Q9UPY3 AB023145 BAA76772 AB028449 BAA78691 AF007142 AJ132261 CAB38857 AK001041 AK001827 AK002007 BAG51002 AK091094 AK091513 BAG52376 AL122105 CAB59269 AL832389 AW297296 AY845867 AAW31883 AY845868 AAW31884 AY929254 AAX18334 AY929255 AAX18335 AY929256 AAX18336 BC046934 BC068591 BC150287 AAI50288 BI913232 BM152875 BP199059 BQ937506 HM595745 ADK25182 KJ175111 AIK25433 XM_011536599 XP_011534901 XM_011536600 XP_011534902 XM_011536601 XP_011534903 XM_011536602 XP_011534904 XM_011536603 XP_011534905 XM_011536604 XP_011534906 XM_011536605 XP_011534907 NM_001195573 NP_001182502 NM_001271282 NP_001258211 NM_001291628 NP_001278557 NM_030621 NP_085124 NM_177438 NP_803187
Function Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA- induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. {ECO:0000269|PubMed:15242644, ECO:0000269|PubMed:15973356, ECO:0000269|PubMed:16142218, ECO:0000269|PubMed:16271387, ECO:0000269|PubMe
Subcellular Location Cytoplasm {ECO:0000269|PubMed:16424907}.
Top Pathways MicroRNAs in cancer

Dicer Antibody - 3363 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Dicer Antibody.

Dicer (D5F2) Rabbit mAb - 5325 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5F2
Western blot analysis of extracts from MCF7 and 293 cells using Dicer (D5F2) Rabbit mAb.

Dicer (D38E7) Rabbit mAb - 5362 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D38E7
Western blot analysis of extracts from MCF7 and 293 cells using Dicer (D38E7) Rabbit mAb.

Dicer (A-2) - sc-136981 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dicer (A-2): sc-136981. Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic staining of ovarian stroma cells.

Dicer (E-7) - sc-393328 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dicer (E-7): sc-393328. Western blot analysis of Dicer expression in NIH/3T3 (A) and KNRK (B) whole cell lysates.

Dicer (C-20) - sc-25117 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dicer (C-20): sc-25117. Western blot analysis of Dicer expression in rat brain tissue extract.

Dicer (E-13) - sc-34600 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Dicer (D-11) - sc-136980 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dicer (D-11): sc-136980. Western blot analysis of human recombinant Dicer enzyme.

Dicer (I-15) - sc-34601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dicer (I-15): sc-34601. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.

Dicer (F-10) - sc-136979 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dicer (F-10): sc-136979. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic and membrane staining of glandular cells.

Dicer (H-212) - sc-30226 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Dicer (H-212): sc-30226. Western blot analysis of Dicer expression in Hep G2 nuclear extract (A) and DT40 whole cell lysate (B).

Dicer (N-14) - sc-34602 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Dicer (13D6) - sc-56651 from Santa Cruz Biotechnology

Host Mouse
Clonality Monoclonal

Anti-Dicer antibody - ab5818 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab5818 (1/200) staining Dicer in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.See Abreview

Anti-Dicer antibody - ab104594 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-Dicer antibody - ab189345 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ELISA analysis of HeLa cell lysate transiently transfected with Dicer or HeLa cells with siRNA, labeling Dicer with ab189345 at 1/200 dilution. 

Anti-Dicer antibody - ab93380 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab93380, at a 1/100 dilution, staining Dicer in the cytoplasm of formalin fixed, paraffin embedded brain tissue by Immunohistochemistry.

Anti-Dicer antibody - ab13502 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab13502 at a 1/1000 dilution staining approximately 217kDa Dicer in 15µg of mouse ovary tissue lysate by western blot (ECL).

Anti-Dicer antibody [13D6] - ChIP Grade - ab14601 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 13D6
Overlay histogram showing HEK293 cells stained with ab14601 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14601, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Dicer antibody [4A6] - ab82539 from Abcam

Prices $378.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 4A6
ab82539, at 1/20 dilution, staining Dicer in normal human ovary by Immunohistochemistry using paraffin-embedded tissue.

Anti-Dicer antibody [S167-7] - ab167444 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S167-7

Anti-DICER1 / Dicer Antibody - LS-C48636 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DICER1 / Dicer Antibody (aa132-150) - LS-C747 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of 15 ug of mouse ovary tissue lysate with anti-mouse Dicer at 1:1000 dilution.

Anti-DICER1 / Dicer Antibody (aa1008-1022) - LS-C147930 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-DICER1 / Dicer Antibody (aa125-142) - LS-C147957 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
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