Anti-Dicer antibody

• Supplier: Abcam
• Catalog: ab13502
• Prices: $382.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (Mouse), corresponding to amino acids FTKHQVLIMTCYVALTVLK (132-150) and QNKQILVPELCAIHPI (1017 -1033)
• Description: Rabbit Polyclonal
• Gene: DICER1
• Conjugate: Unconjugated

Product images

ab13502 at a 1/1000 dilution staining approximately 217kDa Dicer in 15µg of mouse ovary tissue lysate by western blot (ECL).

Anti-Dicer antibody (ab13502) at 1/1000 dilution + Human HeLa cell, whole cell lysate.SecondaryHRP conjugated goat anti-rabbitdeveloped using the ECL techniquePerformed under non-reducing conditions.Observed band size : 218 kDa (why is the actual band size different from the predicted?)Additional bands at : 70 kDa (possible non-specific binding).Exposure time : 3 minutesThis image is courtesy of an Abreview submitted by Dr Andrey PoleshkoSee Abreview

ICC/IF image of ab13502 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13502, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Dicer knockdown inhibits endothelial cell tumor growth via microRNA 21a-3p - Dicer knockdown inhibits endothelial cell tumor growth via microRNA 21a-3p

Gordillo GM, Biswas A, Khanna S, Pan X, Sinha M, Roy S, Sen CK. J Biol Chem. 2014 Mar 28;289(13):9027-38.

Prognostic value of Dicer expression in human breast cancers and association with - Prognostic value of Dicer expression in human breast cancers and association with

Grelier G, Voirin N, Ay AS, Cox DG, Chabaud S, Treilleux I, Leon-Goddard S, Rimokh R, Mikaelian I, Venoux C, Puisieux A, Lasset C, Moyret-Lalle C. Br J Cancer. 2009 Aug 18;101(4):673-83.

Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart - Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart

Chen JF, Murchison EP, Tang R, Callis TE, Tatsuguchi M, Deng Z, Rojas M, Hammond SM, Schneider MD, Selzman CH, Meissner G, Patterson C, Hannon GJ, Wang DZ. Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):2111-6. doi:

Identification of cellular proteins that maintain retroviral epigenetic - Identification of cellular proteins that maintain retroviral epigenetic

Poleshko A, Palagin I, Zhang R, Boimel P, Castagna C, Adams PD, Skalka AM, Katz RA. J Virol. 2008 Mar;82(5):2313-23. Epub 2007 Dec 19.

MicroRNA regulation of cyclooxygenase-2 during embryo implantation. - MicroRNA regulation of cyclooxygenase-2 during embryo implantation.

Chakrabarty A, Tranguch S, Daikoku T, Jensen K, Furneaux H, Dey SK. Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):15144-9. Epub 2007 Sep 11.

miRNA and Dicer in the mammalian lens: expression of brain-specific miRNAs in the - miRNA and Dicer in the mammalian lens: expression of brain-specific miRNAs in the

Frederikse PH, Donnelly R, Partyka LM. Histochem Cell Biol. 2006 Jul;126(1):1-8. Epub 2006 Jan 6.

Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and - Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and

Lugli G, Larson J, Martone ME, Jones Y, Smalheiser NR. J Neurochem. 2005 Aug;94(4):896-905.