Cytochrome-C Oxidase (Complex IV) Human Profiling ELISA Kit
| Name | Cytochrome-C Oxidase (Complex IV) Human Profiling ELISA Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab124538 |
| Category | ELISA Kit |
| Assay Type | Sandwich (qualitative) |
| Sample Type | Tissue Extracts, Cell Lysate |
| Detection | Colorimetric |
| Sensitivity | 20 µg/ml |
| Range | 5 µg/ml - 1000 µg/ml |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Human |
| Gene | COX1 |
| Supplier Page | Shop |
Product images
Example control sample curve of serially titrated HepG2 extract in the working range of the assay.
Human HepG2 cells were cultured in NARTI Zalcitabine (ddC) for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation. The drug reduced mitochondrial DNA levels and hence mitochondrial protein expression. As a consequence the assembly of Complexes I, III and IV were severely affected. Note that loss of the two small mitochondrial DNA encoded subunits of Complex V (ATP synthase) does not affect overall assembly. Interestingly an increase in Complex II was induced as a consequence of I, III, IV loss possibly to up regulate mitochondrial citric acid cycle function.
Human HepG2 cells were cultured in chloramphenicol for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation. The antibiotic inhibited mitochondrial protein translation and assembly of Complexes I and IV but had no significant effect on Complex II, III or V.
Product References
Oxidative phosphorylation differences between mitochondrial DNA haplogroups - Oxidative phosphorylation differences between mitochondrial DNA haplogroups
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2-D DIGE analyses of enriched secretory lysosomes reveal heterogeneous profiles - 2-D DIGE analyses of enriched secretory lysosomes reveal heterogeneous profiles
Schmidt H, Gelhaus C, Nebendahl M, Lettau M, Watzl C, Kabelitz D, Leippe M, Janssen O. Proteomics. 2008 Jul;8(14):2911-25.
Retinoschisin is a peripheral membrane protein with affinity for anionic - Retinoschisin is a peripheral membrane protein with affinity for anionic
Vijayasarathy C, Takada Y, Zeng Y, Bush RA, Sieving PA. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):991-1000.