![PhosphoTracer ERK 1/2 (pT202/Y204) + total ERK ELISA Kit (ab119659) used in Sandwich ELISA.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/1034_ERK-12-phospho-Thr202Tyr204-total-ERK-Kits-ab119659-6.jpg)
PhosphoTracer ERK 1/2 (pT202/Y204) + total ERK ELISA Kit (ab119659) used in Sandwich ELISA.
![Using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/1035_ERK-12-phospho-Thr202Tyr204-total-ERK-Kits-ab119659-5.jpg)
Using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.
![Using Western blot, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/1036_ERK-12-phospho-Thr202Tyr204-total-ERK-Kits-ab119659-4.jpg)
Using Western blot, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.