MAPK3

Gene Symbol MAPK3
Entrez Gene 5595
Alt Symbol ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase 3
Other Description MAP kinase isoform p44|MAPK 1|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1|insulin-stimulated MAP2 kinase|microtubule-associated protein 2 kinase
Swissprots Q8NHX1 P27361 A8CZ58 B0LPG3
Accessions ABY87542 AGC09597 CBK51145 EAW79912 EAW79913 EAW79914 EAW79915 EAW79916 EAW79917 P27361 AK091009 BAG52261 AK307995 AY033607 AAK52329 AY033608 AAK52330 BC000205 AAH00205 BC013992 AAH13992 BX537897 CAD97888 DA351318 DQ399291 ABD60302 DQ893440 ABM84366 DQ896778 ABM87777 KJ534878 AHW56518 M84490 AAA36142 X60188 CAA42744 Z11696 CAA77754 XR_243293 NM_001040056 NP_001035145 NM_001109891 NP_001103361 NM_002746 NP_002737
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organel
Subcellular Location Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization.
Top Pathways TGF-beta signaling pathway, Adherens junction, Axon guidance, VEGF signaling pathway, Focal adhesion

PathScan® Phospho-p44 MAPK (Thr202/Tyr204) Sandwich ELISA Kit - 7315 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 2. The relationship between the protein concentration of the lysate from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm is shown.

PathScan ® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Antibody Pair - 7246 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between the protein concentration of the lysate from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan ® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Antibody Pair #7246 is shown. NIH/3T3 cells were treated with PDGF (100 ng/mL) for 5 minutes at 37ºC and then lysed.

PathScan ® Phospho-p44 MAPK (Thr202/Tyr204) Sandwich ELISA Antibody Pair - 7278 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan® Phospho-p44 MAPK (Thr202/Tyr204) Sandwich ELISA Antibody Pair #7278 is shown. NIH/3T3 cells were treated with PDGF for 5 minutes at 37ºC and then lysed.

PathScan ® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit - 7177 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. Treatment of NIH/3T3 cells with PDGF stimulates phosphorylation of p44/42 MAPK at Thr202/Tyr204, detected by the PathScan ® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit #7177, but does not affect the level of p44/42 MAPK detected by western analysis. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots, using p44/42 MAPK (Erk1/2) Antibody #9102 (left) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb #4370 (right), are shown in the bottom figure.

PathScan ® Total p44/42 MAPK (Erk1/2) Sandwich ELISA Kit - 7050 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. Treatment of NIH/3T3 cells with PDGF stimulates phosphorylation of p44/42 MAPK at Thr202/Tyr204, detected by the PathScan ® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit #7177, but has little effect on the total level of p44/42 MAPK detected by PathScan ® Total p44/42 MAPK (Erk1/2) Sandwich ELISA Kit. NIH/3T3 cells (80-90% confluent) were starved overnight and treated with 100 ng/mL PDGF for 5 minutes at 37ºC. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots, using p44/42 MAPK (Erk1/2) Antibody #9102 (left panel) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb #4370 (right panel), are shown in the bottom figure.

PathScan® Total p44/42 MAPK Sandwich ELISA Kit - 7320 from Cell Signaling Technology

Category ELISA Kit

p44/42 MAP Kinase Assay Kit (Nonradioactive) - 9800 from Cell Signaling Technology

Category Assay Kit
Prices $409.00
Sizes 40 assays
p44/42 MAP Kinase-induced phosphorylation of Elk-1 was measured by immunoblotting with Phospho-Elk-1 (Ser 383)9186, mouse mAb. Lysate titrations were run by immunoprecipitating various amounts of Phospho-p44/42 from 3T3, PDGF lysates and 3T3, U0126 lysates. Phosphorylation of Elk-1 (Ser383) is clearly seen in the positive control lysate compared with the same amounts of the negative control.
Using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation at Thr202/Tyr204 is detected in A431 cells treated with AG1478 for 3 hours (-), compared with cells treated with EGF for 10 min (+).

ERK 1/2 (pT202/Y204) + Total SimpleStep ELISA™ Kit - ab176660 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Example of a typical ERK1/2 (pT202/Y204) and ERK1/2 (Total) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.

ERK1 (MAPK3) Human SimpleStep ELISA™ Kit - ab173195 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 0.86 ng/ml
Range 3.13 ng/ml - 200 ng/ml
Background-subtracted data values (mean +/- SD) are graphed.

Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit - ab126445 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant Human EGF at 37&degC for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126445.

Erk1 (pT202/pY204) + Erk2 (pT185/pY187) ELISA Kit - ab126444 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant Human EGF at 37&degC for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126444.

ERK1/2 (pT202/Y204) +p38 MAPK (pT180/Y182)+JNK1/2/3 (pT183/Y185) PhosphoTracer ELISA Kit - ab119674 from Abcam

Category ELISA Kit
Prices $573.00
Sizes 1 x 96 tests
Using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation at Thr202/Tyr204 is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.

ERK1/2 (Thr202/Tyr204) In-Cell ELISA Kit - ab126423 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were stimulated by different concentrations of EGF for 10 min at 37°C. 

ERK1/2 pThr202/Tyr204 + Total PhosphoTracer ELISA Kit - ab119659 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
PhosphoTracer ERK 1/2 (pT202/Y204) + total ERK ELISA Kit (ab119659) used in Sandwich ELISA.

Mouse/Human/Rat MAPK3 / ERK1 ELISA Kit (Cell-Based ELISA) - LS-F3217 from LifeSpan Bioscience

Category ELISA Kit
Prices $450.00
Sizes 1 plate
Range 0.313-20 ng/ml

Human MAPK3 / ERK1 ELISA Kit (Sandwich ELISA) - LS-F4571 from LifeSpan Bioscience

Category ELISA Kit
Prices $690.00
Sizes 1 plate
Sensitivity 0.313 ng/ml
Range 0.313-20 ng/ml

Mouse/Human/Rat Phospho-MAPK3 / ERK1 (Thr202/Tyr204) ELISA Kit - LS-F544 from LifeSpan Bioscience

Category ELISA Kit
Prices $540.00, $950.00, $2,180.00
Sizes 1 plate, 2 plate, 5 plate
Range 0.313-20 ng/ml

Rat MAPK3 / ERK1 ELISA Kit (Sandwich ELISA) - LS-F9955 from LifeSpan Bioscience

Category ELISA Kit
Prices $740.00
Sizes 1 plate
Sensitivity 0.258 ng/ml
Range 0.313-20 ng/ml

Rabbit anti-ERK1 Antibody, Affinity Purified - PLA0235 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunohistochemistry</B><BR/>Rabbit Anti-ERK1 Antibody, Affinity Purified: <B>Cat. No. PLA0235</B>: Detection of Human ERK1 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-ERK1 (<B>Cat. No. PLA0235</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Phospho-Erk1 (pThr202 / pTyr204 + Erk2 (pTyr185/187) and pan-Erk1 / 2 ELISA Kit - RAB0349 from Sigma-Aldrich

Category ELISA Kit
Prices $570.00
Sizes 1 kt

Phospho-Erk1 (pThr202 / pTyr204) + Erk2(pTyr185/187) ELISA Kit - RAB0348 from Sigma-Aldrich

Category ELISA Kit
Prices $522.00
Sizes 1 kt

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Cell-Based ELISA - KCB1018 from R&D Systems

Category ELISA Kit
Prices $459.00
Sizes 1 kit

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA - DYC1018B-2 from R&D Systems

Category ELISA Kit
Prices $329.00, $649.00, $1,395.00
Sizes 1 kit (for 2 Plates), 1 kit (for 5 Plates), 1 pack (for 15 Plates)
Range 469.00 - 30,000 pg/mL
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