Rabbit anti-PCNA Antibody, Affinity Purified

Name	Rabbit anti-PCNA Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0079
Prices	$323.50
Sizes	100 µl
Applications	FC
Species Reactivities	Human, Rabbit, Bovine, Guinea Pig, Dog, Rat, Chicken, Pig, Turkey, Mouse, Horse
Gene	PCNA
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-PCNA Antibody, Affinity Purified: <B>Cat. No. PLA0079</B>: Detection of Human PCNA by Immunohistochemistry. Sample: FFPE section of Human stomach carcinoma. Antibody: Affinity purified Rabbit Anti-PCNA (<B>Cat. No. PLA0079</B>) used at a dilution of 1:10,000 (0.1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-PCNA Antibody, Affinity Purified: Cat. No. PLA0079: Detection of Human PCNA by Immunohistochemistry. Sample: FFPE section of Human stomach carcinoma. Antibody: Affinity purified Rabbit Anti-PCNA (Cat. No. PLA0079) used at a dilution of 1:10,000 (0.1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-PCNA Antibody, Affinity Purified: <B>Cat. No. PLA0079</B>: Detection of Human PCNA by Western Blot and Immunoprecipitaiton. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 μg for IP; 30 μg for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified Rabbit Anti-PCNA Antibody (<B>Cat. No. PLA0079</B>) used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using Antibodies recognizing different epitopes (including Cat. No. <B>PLA0080</B>) or a Mouse monoclonal Antibody to PCNA (mAb). Control mock IP was performed using normal Mouse IgG (M-IgG) and normal Rabbit IgG (R-IgG). Immunoprecipitated PCNA was blotted using one of the alternate Antibodies (each at 1 μg/mL). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).

Immunoblotting
Rabbit Anti-PCNA Antibody, Affinity Purified: Cat. No. PLA0079: Detection of Human PCNA by Western Blot and Immunoprecipitaiton. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 μg for IP; 30 μg for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified Rabbit Anti-PCNA Antibody (Cat. No. PLA0079) used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using Antibodies recognizing different epitopes (including Cat. No. PLA0080) or a Mouse monoclonal Antibody to PCNA (mAb). Control mock IP was performed using normal Mouse IgG (M-IgG) and normal Rabbit IgG (R-IgG). Immunoprecipitated PCNA was blotted using one of the alternate Antibodies (each at 1 μg/mL). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).

<B>Flow Cytometry</B><BR/>Rabbit Anti-PCNA Antibody, Affinity Purified: <B>Cat. No. PLA0079</B>: Flow Cytometric Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% Triton-X-100, 0.5 mM EDTA, 1% BSA) and 100% methanol. 1 × 10<SUP>6</SUP> cells were stained with 0.03 μg Anti-PCNA (<B>PLA0079</B>). Secondary detection was performed with FITC conjugated Goat F(ab′)2 Anti-Rabbit Antibody, and DNA stained with PI.

Flow Cytometry
Rabbit Anti-PCNA Antibody, Affinity Purified: Cat. No. PLA0079: Flow Cytometric Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% Triton-X-100, 0.5 mM EDTA, 1% BSA) and 100% methanol. 1 × 10⁶ cells were stained with 0.03 μg Anti-PCNA (PLA0079). Secondary detection was performed with FITC conjugated Goat F(ab′)2 Anti-Rabbit Antibody, and DNA stained with PI.