Rabbit anti-FOXO3a Antibody, Affinity Purified

Name	Rabbit anti-FOXO3a Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0107
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Bovine, Sheep, Dog, Primate, Monkey, Ape, Mouse, Human, Horse, Pig, Chimpanzee
Gene	FOXO3
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-FOXO3a Antibody, Affinity Purified: <B>Cat. No. PLA0107</B>: Detection of Human and Mouse FOXO3a by Immunohistochemistry. Sample: FFPE sections of Human ovarian carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-FOXO3a (<B>Cat. No. PLA0107</B>) used at a dilution of 1:500 (2 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-FOXO3a Antibody, Affinity Purified: Cat. No. PLA0107: Detection of Human and Mouse FOXO3a by Immunohistochemistry. Sample: FFPE sections of Human ovarian carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-FOXO3a (Cat. No. PLA0107) used at a dilution of 1:500 (2 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-FOXO3a Antibody, Affinity Purified: <B>Cat. No. PLA0107</B>: Detection of Human and Mouse FOXO3a by Western Blot. Samples: Whole cell lysate (50 μg) from 293T, Jurkat, and Mouse TCMK-1 cells. Antibodies: Affinity purified Rabbit Anti-FOXO3a Antibody <B>PLA0107</B> used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-FOXO3a Antibody, Affinity Purified: Cat. No. PLA0107: Detection of Human and Mouse FOXO3a by Western Blot. Samples: Whole cell lysate (50 μg) from 293T, Jurkat, and Mouse TCMK-1 cells. Antibodies: Affinity purified Rabbit Anti-FOXO3a Antibody PLA0107 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-FOXO3a Antibody, Affinity Purified: <B>Cat. No. PLA0107</B>: Detection of Human FOXO3a by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF7 cells. Antibodies: Affinity purified Rabbit Anti-FOXO3a antibody <B>Cat. No. PLA0107</B> used for IP at 6 μg/mg lysate. FOXO3a was also immunoprecipitated by Rabbit Anti-FOXO3a antibody B. For blotting immunoprecipitated FOXO3a, <B>PLA0107</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation
Rabbit Anti-FOXO3a Antibody, Affinity Purified: Cat. No. PLA0107: Detection of Human FOXO3a by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF7 cells. Antibodies: Affinity purified Rabbit Anti-FOXO3a antibody Cat. No. PLA0107 used for IP at 6 μg/mg lysate. FOXO3a was also immunoprecipitated by Rabbit Anti-FOXO3a antibody B. For blotting immunoprecipitated FOXO3a, PLA0107 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.