![A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3292_STAT1-pY701-total-STAT1-Kits-ab126457-6.jpg)
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
![A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed using this phosphoELISA.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3293_STAT1-pY701-total-STAT1-Kits-ab126457-7.jpg)
A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed using this phosphoELISA.
![A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed using Western Blot.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3294_STAT1-pY701-total-STAT1-Kits-ab126457-11.jpg)
A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed using Western Blot.
![The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes to induce phosphorylation of STAT1. Serial dilutions of lysates were analyzed in this ELISA.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3295_STAT1-pY701-total-STAT1-Kits-ab126457-9.jpg)
The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes to induce phosphorylation of STAT1. Serial dilutions of lysates were analyzed in this ELISA.
![The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes to induce phosphorylation of STAT1. Serial dilutions of lysates were analyzed by Western blot. Immunoblots were incubated with anti-phospho-STAT1 (Tyr701).](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3296_STAT1-pY701-total-STAT1-Kits-ab126457-12.jpg)
The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes to induce phosphorylation of STAT1. Serial dilutions of lysates were analyzed by Western blot. Immunoblots were incubated with anti-phospho-STAT1 (Tyr701).