![A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126456.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3297_STAT1-phospho-Tyr701-Kits-ab126456-6.jpg)
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126456.
![A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 10 min. Cell lysates were analyzed using ab126456.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3298_STAT1-phospho-Tyr701-Kits-ab126456-2.JPG)
A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 10 min. Cell lysates were analyzed using ab126456.
![A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 10 min. Cell lysates were analyzed by Western Blot.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3299_STAT1-phospho-Tyr701-Kits-ab126456-9.jpg)
A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 10 min. Cell lysates were analyzed by Western Blot.
![A431 cells were treated with 100 ng/mL recombinant Human EGF for 20 minutes to induce phosphorylation of EGF R. Serial dilutions of lysates were analyzed using ab126456.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3300_STAT1-phospho-Tyr701-Kits-ab126456-4.JPG)
A431 cells were treated with 100 ng/mL recombinant Human EGF for 20 minutes to induce phosphorylation of EGF R. Serial dilutions of lysates were analyzed using ab126456.
![The A431 cells were treated with 100 ng/mL recombinant Human EGF for 20 minutes to induce phosphorylation of EGF R. Serial dilutions of lysates were analyzed by western blot. Immunoblots were incubated with anti-phospho-STAT1 (Tyr701).](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/3301_STAT1-phospho-Tyr701-Kits-ab126456-8.jpg)
The A431 cells were treated with 100 ng/mL recombinant Human EGF for 20 minutes to induce phosphorylation of EGF R. Serial dilutions of lysates were analyzed by western blot. Immunoblots were incubated with anti-phospho-STAT1 (Tyr701).