Example of a typical STAT1 (pY701) cell lysate dilution series. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1 x Cell Extraction Buffer PTR. Data from duplicate measurements of STAT1 (pY701) are normalized and plotted.
Induction of STAT1 (pY701) phosphorylation in HeLa cells in response to IFN-gamma treatment. HeLa cells were cultured in 96-well tissue culture plates, serum-starved and treated (20 min) with a dose-range of IFN-gamma before cell lysis. Data from quadruplicate measurements of STAT1 (pY701) are plotted. Comparative STAT1 (pY701) and STAT1 (Total) data also shown by Western Blot.